Nikos Hatzakis
Professor
Department of Chemistry
Universitetsparken 5
2100 København Ø
POLY 391-Assembly strategies for enzyme immobilization
Lambermon, M., Hendriks, L. J., Schoffelen, S., Vos, M., Hatzakis, Nikos, Carette, N., Rowan, A. E., Cornelissen, J., Michon, T., Sommerdijk, N. A. J. M. & van Hest, J. C. M., 2006, In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Research output: Contribution to journal › Conference abstract in journal › Research
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Synergy between membrane-curvature and liquid ordered phase-state effectively sorts amphiphilic protein-anchoring motifs
Larsen, J., Jensen, M. B., Hatzakis, Nikos, Bhatia, V. K. & Stamou, Dimitrios, 2011, In: European Biophysics Journal. 40, Suppl 1, p. S200 1 p.Research output: Contribution to journal › Conference abstract in journal › Research
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Observation of Inhomogeneity in the Lipid Composition of Individual Nanoscale Liposomes
Larsen, J., Hatzakis, Nikos, Elizondo, E., Cabrera, I., Veciana, J., Ventosa, N. & Stamou, Dimitrios, 2012, In: Biophysical Journal. p. 426a 1 p.Research output: Contribution to journal › Conference abstract in journal › Research
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Observation of inhomogeneity in the lipid composition of individual nanoscale liposomes
Larsen, J., Hatzakis, Nikos & Stamou, Dimitrios, 20 Jul 2011, In: Journal of the American Chemical Society. 133, 28, p. 10685-10687 3 p.Research output: Contribution to journal › Letter › Research › peer-review
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Lipid-Anchored Ras is Sorted by Membrane Curvature Both In Vitro and in Living Cells
Larsen, J., Jensen, M. B., Bhatia, V. K., Pedersen, S. L., Bjornholm, T., Jensen, Knud Jørgen, Kohnke, M., Abankwa, D., Alexandrov, K., Hatzakis, Nikos & Stamou, Dimitrios, 2013, In: Biophysical Journal. 104, 2, 1 p., 500-Pos.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
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Membrane Curvature and Lipid Composition Synergize To Regulate N-Ras Anchor Recruitment
Larsen, J. B., Kennard, C., Pedersen, S. L., Jensen, Knud Jørgen, Uline, M. J., Hatzakis, Nikos & Stamou, Dimitrios, 2017, In: Biophysical Journal. 113, 6, p. 1269-1279 11 p.Research output: Contribution to journal › Journal article › Research › peer-review
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How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature
Larsen, J. B., Rosholm, K. R., Kennard, C., Pedersen, S. L., Munch, H. K., Tkach, Vadym, Sakon, J. J., Bjornholm, T., Weninger, K. R., Bendix, Poul Martin, Jensen, Knud Jørgen, Hatzakis, Nikos, Uline, M. J. & Stamou, Dimitrios, 2020, In: ACS Central Science. 6, 7, p. 1159-1168 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
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N-RAS lipid anchor adsorption to membranes as a function of lipid composition and curvature
Larsen, J. B., Kennard, C., Pedersen, S. L., Jensen, Knud Jørgen, Hatzakis, Nikos, Uline, M. J. & Stamou, Dimitrios, 2016, In: Biophysical Society. Annual Meeting. Abstracts. 110, 3, p. 579A 1 p.Research output: Contribution to journal › Conference abstract in journal › Research
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Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases
Larsen, J. B., Jensen, M. B., Bhatia, V. K., Pedersen, S. L., Bjørnholm, T., Iversen, L., Uline, M., Szleifer, I., Jensen, Knud Jørgen, Hatzakis, Nikos & Stamou, Dimitrios, 2015, In: Nature Chemical Biology. 11, 3, p. 192-194 3 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Single molecule activity measurements of cytochrome P450 oxidoreductase reveal the existence of two discrete functional states
Laursen, Tomas, Singha, A., Rantzau, N., Tutkus, M., Borch-Jensen, J., Hedegård, Per, Stamou, Dimitrios, Møller, Birger Lindberg & Hatzakis, Nikos, 2014, In: ACS chemical biology. 9, 3, p. 630-634 5 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum
Laursen, Tomas, Borch-Jensen, J., Baden, C. K., Bavishi, K., Torta, F., Martens, Helle Jakobe, Silvestro, D., Hatzakis, Nikos, Wenk, M. R., Dafforn, T. R., Olsen, C. E., Motawie, Mohammed Saddik, Hamberger, B. R., Møller, Birger Lindberg & Bassard, J. A., 2016, In: Science (New York, N.Y.). 354, 6314, p. 890-893 4 p.Research output: Contribution to journal › Journal article › Research › peer-review
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FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Lerner, E., Barth, A., Hendrix, J., Ambrose, B., Birkedal, V., Blanchard, S. C., Börner, R., Chung, H. S., Cordes, T., Craggs, T. D., Deniz, A. A., Diao, J., Fei, J., Gonzalez, R. L., Gopich, I. V., Ha, T., Hanke, C. A., Haran, G., Hatzakis, N. S., Hohng, S. & 27 others, , 2021, In: eLife. 10, 69 p., e60416.Research output: Contribution to journal › Review › Research › peer-review
- Published
Single enzyme experiments reveal a long-lifetime proton leak state in a heme-copper oxidase
Li, M., Jørgensen, S. K., McMillan, D. G. G., Krzemiński, Ł., Daskalakis, N. N., Partanen, R. H., Tutkus, M., Tuma, R., Stamou, Dimitrios, Hatzakis, Nikos & Jeuken, L. J. C., 2015, In: Journal of the American Chemical Society. 137, 51, p. 16055-16063 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Effects of membrane curvature and pH on proton pumping activity of single cytochrome bo3 enzymes
Li, M., Khan, S., Rong, H., Tuma, R., Hatzakis, Nikos & Jeuken, L. J. C., 2017, In: Biochimica et Biophysica Acta - Bioenergetics. 1858, 9, p. 763-770Research output: Contribution to journal › Journal article › Research › peer-review
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Single-particle combinatorial multiplexed liposome fusion mediated by DNA
Malle, M., Loffler, P., Bohr, S. S., Sletfjerding, M., Risgaard, N., Jensen, S., Zhang, Min, Hedegård, Per, Vogel, S. & Hatzakis, Nikos, 2022, In: Nature Chemistry. 14, 5, p. 558–565 18 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Mazurenko, I., Hatzakis, Nikos & Jeuken, L. J. C., 2019, In: Journal of Visualized Experiments. 144Research output: Contribution to journal › Journal article › Research › peer-review
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A systematic analysis of regression models for protein engineering
Michael, Richard, Kæstel-Hansen, Jacob, Groth, Peter Mørch, Bartels, S., Salomon, J., Tian, P., Hatzakis, Nikos & Boomsma, Wouter, 2024, In: PLOS Computational Biology. 20, 5 May, 22 p., e1012061.Research output: Contribution to journal › Journal article › Research › peer-review
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Physical and barrier changes in gastrointestinal mucus induced by the permeation enhancer sodium 8-[(2-hydroxybenzoyl)amino]octanoate (SNAC)
Mortensen, Janni Støvring, Bohr, S. S. R., Rønholt, Stine, Hatzakis, Nikos, Saaby, Lasse & Nielsen, Hanne Mørck, 2022, In: Journal of Controlled Release. 352, p. 163-178 16 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Neonatal intestinal mucus barrier changes in response to maturity, inflammation, and sodium decanoate supplementation
Mortensen, Janni Støvring, Bohr, S. S. R., Krog, Lasse Skjoldborg, Bøtker, Johan Peter, Kapousidou, V., Saaby, Lasse, Hatzakis, Nikos, Nielsen, Hanne Mørck, Nguyen, Duc Ninh & Rønholt, Stine, 2024, In: Scientific Reports. 14, 1, 17 p., 7665.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Single-Molecule Study of Thermomyces lanuginosus Lipase in a Detergency Application System Reveals Diffusion Pattern Remodeling by Surfactants and Calcium
Moses, M. E., Lund, P. M., Bohr, S. S., Iversen, J. F., Kæstel-Hansen, J., Kallenbach, A. S., Iversen, L., Christensen, S. M. & Hatzakis, Nikos, 2021, In: A C S Applied Materials and Interfaces. 13, 28, 9 p., 33704.Research output: Contribution to journal › Journal article › Research › peer-review
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Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout
Moses, M. E., Hedegård, Per & Hatzakis, Nikos, 2016, Single-molecule enzymology: fluorescence-based and high-throughput methods. Spies, M. & Chemla, Y. R. (eds.). Academic Press, p. 227-256 30 p. (Methods in Enzymology, Vol. 581).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Links of conformational sampling to functional plasticity and clinical phenotypes by single molecule studies
Moses, M. E., Thodberg, S., Bavishi, K., Eiersholt, S., Li, D., Stamou, Dimitrios, Møller, Birger Lindberg, Laursen, Tomas & Hatzakis, Nikos, 2016, In: Biophysical Society. Annual Meeting. Abstracts. 110, 3, p. 397A 1 p.Research output: Contribution to journal › Conference abstract in journal › Research
- Published
Optically Induced Linking of Protein and Nanoparticles to Gold Surfaces
Moth-Poulsen, K., Kofod-Hansen, V., S. Kamounah, F., Hatzakis, Nikos, Stamou, Dimitrios, Schaumburg, K. & Christensen, Jørn Bolstad, 2010, In: Bioconjugate Chemistry. 21, p. 1056 1061 p.Research output: Contribution to journal › Journal article › Research › peer-review
POLY 553-Biohybrid block copolymer architectures by "click" chemistry
Opsteen, J. A., Dirks, A. J., van Berkel, S. S., Hatzakis, Nikos, Brinkhuis, R. P., Reijnders, M. E. G. & van Hest, J. C. M., 2006, In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.Research output: Contribution to journal › Conference abstract in journal › Research
- Published
Single-particle diffusional fingerprinting: A machine-learning framework for quantitative analysis of heterogeneous diffusion
Pinholt, H. D., Bohr, S. S. R., Iversen, J. F., Boomsma, Wouter & Hatzakis, Nikos, 2021, In: Proceedings of the National Academy of Sciences of the United States of America. 118, 31, 7 p., e2104624118.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells
Rosholm, K. R., Leijnse, Natascha, Mantsiou, A., Tkach, Vadym, Pedersen, S. L., Wirth, V. F., Oddershede, L. B., Jensen, Knud Jørgen, Martinez, Karen Laurence, Hatzakis, Nikos, Bendix, Poul Martin, Callan-Jones, A. & Stamou, Dimitrios, 8 May 2017, In: Nature Chemical Biology. 13, 7, p. 724-729Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Sorting of tN-Ras by Membrane Curvature in Lipid Vesicles and Tubes
Rosholm, K. R., Larsen, J., Pedersen, S. L., Munch, H. K., Jensen, Knud Jørgen, Hatzakis, Nikos & Stamou, Dimitrios, 2013, In: Biophysical Journal. 104, Supplement 1, p. 549A 1 p.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
- Published
The dopamine transporter antiports potassium to increase the uptake of dopamine
Schmidt, S. G., Malle, M. G., Nielsen, A. K., Bohr, S. S., Pugh, Ciara Frances, Nielsen, Jeppe Cederholm, Poulsen, I. H., Rand, Kasper Dyrberg, Hatzakis, Nikos & Loland, Claus Juul, 2022, In: Nature Communications. 13, 1, 12 p., 2446.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Direct Observation of Sophorolipid Micelle Docking in Model Membranes and Cells by Single Particle Studies Reveals Optimal Fusion Conditions
Singh, P. K., Bohr, S. S. & Hatzakis, Nikos, 2020, In: Biomolecules. 10, 9, 16 p., 1291.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Membrane Curvature Regulates the Localization of G Protein Coupled Receptors and Ras Isoforms
Stamou, Dimitrios, Rosholm, K. R., Damalas, A., Hatzakis, Nikos, Wirth, V. F., Martinez, Karen Laurence, Leijnse, Natascha, Oddershede, L. B., Bendix, Poul Martin & Pedersen, S. L., 27 Jan 2015, In: Biophysical Journal. 108, 2, p. 95a-96aResearch output: Contribution to journal › Journal article › Research › peer-review
- Published
Static and Dynamic Disorder Observed in the Phase Transition Behavior of Individual Small Unilamellar Vesicles
Stamou, Dimitrios, Bendix, Poul Martin, Wibroe, P. P. & Hatzakis, Nikos, 2011, In: Biophysical Journal. 100, 3 suppl 1, p. 35a 1 p.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
- Published
Direct Observation of CRISPR-Cas12 Conformational Sampling by SM FRET and Cryo EM Reveals how Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity
Stella, S., Mesa, Pablo, Thomsen, J., Paul, B., Alcon, P., Jensen, S. B., Moses, M. E., Montoya, Guillermo & Hatzakis, Nikos, 7 Feb 2020, In: Biophysical Journal. 118, 3, p. 223A-224A 2 p.Research output: Contribution to journal › Conference abstract in journal › Research
- Published
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
Stella, S., Mesa, Pablo, Thomsen, J., Paul, B., Alcón, P., Jensen, S. B., Saligram, B., Moses, M. E., Hatzakis, Nikos & Montoya, Guillermo, 13 Dec 2018, In: Cell. 175, 7, p. 1856-1871.e21 16 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Interactions of Cell-Penetrating Peptide-Modified Nanoparticles with Cells Evaluated Using Single Particle Tracking
Streck, S., Bohr, S. S., Birch, D., Rades, Thomas, Hatzakis, Nikos, McDowell, A. & Nielsen, Hanne Mørck, 2021, In: ACS Applied Bio Materials. 4, 4, p. 3155-3165 11 p.Research output: Contribution to journal › Journal article › Research › peer-review
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DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning
Thomsen, J., Sletfjerding, M. B., Jensen, S. B., Stella, S., Paul, B., Malle, M. G., Montoya, Guillermo, Petersen, Troels Christian & Hatzakis, Nikos, Nov 2020, In: eLife. 9, 21 p., e60404.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
A large size-selective DNA nanopore with sensing applications
Thomsen, R. P., Malle, M. G., Okholm, A. H., Krishnan, S., Bohr, S. S., Sørensen, R. S., Ries, O., Vogel, S., Simmel, F. C., Hatzakis, Nikos & Kjems, J., 2019, In: Nature Communications. 10, 1Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Morphological integrity of insulin amyloid-like aggregates depends on preparation methods and post-production treatments
Thorlaksen, C., Stanciu, A., Neergaard, M. B., Hatzakis, Nikos, Foderà, Vito & Groenning, M., 2022, In: European Journal of Pharmaceutics and Biopharmaceutics. 179, p. 147-155 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
In vitro and in vivo immunogenicity assessment of protein aggregate characteristics
Thorlaksen, C., Schultz, H. S., Gammelgaard, S. K., Jiskoot, W., Hatzakis, Nikos, Nielsen, F. S., Solberg, H., Foderà, Vito, Bartholdy, C. & Groenning, M., 2023, In: International Journal of Pharmaceutics. 631, 122490.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Probing Activation and Conformational Dynamics of the Vesicle-Reconstituted β2 Adrenergic Receptor at the Single-Molecule Level
Tutkus, M., Lundgaard, C. V., Veshaguri, S., Tønnesen, A., Hatzakis, Nikos, Rasmussen, Søren Gøgsig Faarup & Stamou, Dimitrios, 2024, In: Journal of Physical Chemistry B. 128, 9, p. 2124–2133 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy
Veit, S., Paweletz, L. C., Bohr, S. S., Menon, A. K., Hatzakis, Nikos & Pomorski, Thomas Guenther, 2022, In: ACS applied materials & interfaces. 14, 26, p. 29659−29667 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Resolving active ion transport at the single molecule level for the first time
Veshaguri, S., Christensen, S. M., Kemmer, G. C., Møller, M. P., Ghale, G., Lohr, C., Christensen, A. L., Justesen, B. H., Jørgensen, I. L., Schiller, J., Hatzakis, Nikos, Grabe, M., Pomorski, Thomas Guenther & Stamou, Dimitrios, 2016, In: Biophysical Journal. 110, 3, p. 179A 1 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Direct observation of proton pumping by a eukaryotic P-type ATPase
Veshaguri, S., Christensen, S. F., Kemmer, G. C., Ghale, G., Møller, M. P., Lohr, C., Christensen, A. L., Justesen, B. H., Jørgensen, I. L., Schiller, J., Hatzakis, Nikos, Grabe, M., Pomorski, Thomas Guenther & Stamou, Dimitrios, 2016, In: Science. 351, 6280, p. 1469-1473 5 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Developing an Assay to Probe Activtion and Conformational Dynamics of beta 2-Adrenergic Receptor on Single Molecule Level
Veshaguri, S., Tutkus, M., Lundgaard, C. V., Tonnesen, A., Mathiasen, Signe, Jorgensen, S. K., Iversen, L., Hatzakis, Nikos, Rasmussen, Søren Gøgsig Faarup & Stamou, Dimitrios, 2013, In: Biophysical Journal. 104, 2, 1 p.Research output: Contribution to journal › Conference abstract in journal › Research
Positional assembly of enzymes in polymersome nanoreactors for cascade reactions
Vriezema, D. M., Garcia, P. M. L., Oltra, N. S., Hatzakis, Nikos, Kuiper, S. M., Nolte, R. J. M., Rowan, A. E. & van Hest, J. C. M., 2007, In: Angewandte Chemie International Edition. 46, 39, p. 7378-7382 5 p.Research output: Contribution to journal › Letter › Research › peer-review
- Published
Monitoring shifts in the conformation equilibrium of the membrane protein cytochrome P450 reductase (POR) in nanodiscs
Wadsäter, M. H., Laursen, Tomas, Singha, A., Hatzakis, Nikos, Stamou, Dimitrios, Barker, R., Mortensen, Kell, Feidenhans'l, Robert Krarup, Møller, Birger Lindberg & Cardenas Gomez, M., 2012, In: Journal of Biological Chemistry. 287, 41, p. 34596-34603 8 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs
Wan, F., Bohr, S. S. R., Kłodzińska, S. N., Jumaa, H., Huang, Z., Nylander, T., Thygesen, Mikkel Boas, Sørensen, K. K., Jensen, Knud Jørgen, Sternberg, C., Hatzakis, Nikos & Nielsen, Hanne Mørck, 2020, In: ACS Applied Materials and Interfaces. 12, p. 380-389Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Robust Dual Optical Sensor for pH and Dissolved Oxygen
Wang, L., Jensen, K., Hatzakis, Nikos, Zhang, Min & Sørensen, Thomas Just, 2022, In: ACS Sensors. 7, 5, p. 1506–1513Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Functionalized Gold Nanoclusters Identify Highly Reactive Oxygen Species in Living Organisms
Xie, Y., Xianyu, Y., Wang, N., Yan, Z., Liu, Y., Zhu, K., Hatzakis, Nikos & Jiang, X., 2018, In: Advanced Functional Materials. 28, 14, 7 p., 1702026.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Direct Observation of Anisotropy-Driven Formation of Amyloid Spherulites in Real-time by Super-resolution Microscopy
Zhang, Min, Pinholt, H., Zhou, X., Bohr, S., Banetta, L., Zaccone, A., Foderà, Vito & Hatzakis, Nikos, 2021, 19 p.Research output: Working paper › Preprint › Research
- Published
Direct observation of heterogeneous formation of amyloid spherulites in real-time by super-resolution microscopy
Zhang, Min, Pinholt, H., Zhou, X., Bohr, S., Banetta, L., Zaccone, A., Foderà, Vito & Hatzakis, Nikos, 2022, In: Communications Biology . 5, 1, 10 p., 850.Research output: Contribution to journal › Journal article › Research › peer-review
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Insights in enzyme functional dynamics and activity regulation by single molecule studies
Research output: Contribution to journal › Journal article › Research › peer-review
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Single enzyme experiments reveal a long-lifetime proton leak state in a heme-copper oxidase
Research output: Contribution to journal › Journal article › Research › peer-review
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192
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Links of conformational sampling to functional plasticity and clinical phenotypes by single molecule studies
Research output: Contribution to journal › Conference abstract in journal › Research
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