ZAKβ is activated by cellular compression and mediates contraction-induced MAP kinase signaling in skeletal muscle

Research output: Contribution to journalJournal articlepeer-review

Documents

  • Fulltext

    Final published version, 3.42 MB, PDF document

  • Cathrine Nordgaard
  • Amy Stonadge
  • Pedro Antas
  • Marie Sofie Reinert
  • Dorte Breinholdt Bekker-Jensen
  • Yekaterina A Miroshnikova
  • Abdelghani Mazouzi
  • Sarah Falk
  • Emeline Perrier-Groult
  • Christopher Tiedje
  • Xiang Li
  • Jens Rithamer Jakobsen
  • Nicolas Oldenburg Jørgensen
  • Frederic Mallein-Gerin
  • Jesper Løvind Andersen
  • Cristian Pablo Pennisi
  • Thijn Brummelkamp
  • Vivian Sw Li
  • Sara A Wickström
  • Gonzalo Blanco

Mechanical inputs give rise to p38 and JNK activation, which mediate adaptive physiological responses in various tissues. In skeletal muscle, contraction-induced p38 and JNK signaling ensure adaptation to exercise, muscle repair, and hypertrophy. However, the mechanisms by which muscle fibers sense mechanical load to activate this signaling have remained elusive. Here, we show that the upstream MAP3K ZAKβ is activated by cellular compression induced by osmotic shock and cyclic compression in vitro, and muscle contraction in vivo. This function relies on ZAKβ's ability to recognize stress fibers in cells and Z-discs in muscle fibers when mechanically perturbed. Consequently, ZAK-deficient mice present with skeletal muscle defects characterized by fibers with centralized nuclei and progressive adaptation towards a slower myosin profile. Our results highlight how cells in general respond to mechanical compressive load and how mechanical forces generated during muscle contraction are translated into MAP kinase signaling.

Original languageEnglish
Article numbere111650
JournalE M B O Journal
Volume41
Issue number17
Number of pages19
ISSN0261-4189
DOIs
Publication statusPublished - 2022

Bibliographical note

© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

Number of downloads are based on statistics from Google Scholar and www.ku.dk


No data available

ID: 315182860