GPR183 is Dispensable for B1 cell Accumulation and Function, but Affects B2 cell Abundance, in the Omentum and Peritoneal Cavity

Research output: Contribution to journalJournal articleResearchpeer-review


  • Fulltext

    Final published version, 1.06 MB, PDF document

B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abun-dant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a-or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.

Original languageEnglish
Article number494
Issue number3
Number of pages19
Publication statusPublished - 2022

Bibliographical note

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

    Research areas

  • 7TM receptor, B1 cell, B-1 cell, GPCR, GPR183, EBI2, Oxysterol

Number of downloads are based on statistics from Google Scholar and

No data available

ID: 291363158