Cadmium inhibits human DNA mismatch repair in vivo
Research output: Contribution to journal › Journal article › peer-review
The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repair activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reversed by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMR activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutS alpha and hEXO1-hMutL alpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair activity known to play an important role in colon cancer and that this effect can be reversed by Zn treatment.
Original language | English |
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Journal | Biochemical and Biophysical Research Communications |
Volume | 321 |
Issue number | 1 |
Pages (from-to) | 21-5 |
Number of pages | 5 |
ISSN | 0006-291X |
DOIs | |
Publication status | Published - 13 Aug 2004 |
Externally published | Yes |
- Alkylation, Base Pair Mismatch, Base Sequence, Cadmium Chloride, Carcinogens, Cations, Divalent, Cell Cycle, Cell Line, DNA, DNA Damage, DNA Repair, DNA Repair Enzymes, Deoxyribonucleases, Exodeoxyribonucleases, Humans, Kidney, Kinetics, Oligodeoxyribonucleotides
Research areas
ID: 119639148