Cadmium inhibits human DNA mismatch repair in vivo

Research output: Contribution to journalJournal articleResearchpeer-review

Anne Lützen, Sascha Emilie Liberti, Lene Juel Rasmussen

The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repair activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reversed by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMR activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutS alpha and hEXO1-hMutL alpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair activity known to play an important role in colon cancer and that this effect can be reversed by Zn treatment.

Original languageEnglish
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number1
Pages (from-to)21-5
Number of pages5
ISSN0006-291X
DOIs
Publication statusPublished - 13 Aug 2004
Externally publishedYes

    Research areas

  • Alkylation, Base Pair Mismatch, Base Sequence, Cadmium Chloride, Carcinogens, Cations, Divalent, Cell Cycle, Cell Line, DNA, DNA Damage, DNA Repair, DNA Repair Enzymes, Deoxyribonucleases, Exodeoxyribonucleases, Humans, Kidney, Kinetics, Oligodeoxyribonucleotides

ID: 119639148