Cadmium inhibits human DNA mismatch repair in vivo

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Cadmium inhibits human DNA mismatch repair in vivo. / Lützen, Anne; Liberti, Sascha Emilie; Rasmussen, Lene Juel.

In: Biochemical and Biophysical Research Communications, Vol. 321, No. 1, 13.08.2004, p. 21-5.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Lützen, A, Liberti, SE & Rasmussen, LJ 2004, 'Cadmium inhibits human DNA mismatch repair in vivo', Biochemical and Biophysical Research Communications, vol. 321, no. 1, pp. 21-5. https://doi.org/10.1016/j.bbrc.2004.06.102

APA

Lützen, A., Liberti, S. E., & Rasmussen, L. J. (2004). Cadmium inhibits human DNA mismatch repair in vivo. Biochemical and Biophysical Research Communications, 321(1), 21-5. https://doi.org/10.1016/j.bbrc.2004.06.102

Vancouver

Lützen A, Liberti SE, Rasmussen LJ. Cadmium inhibits human DNA mismatch repair in vivo. Biochemical and Biophysical Research Communications. 2004 Aug 13;321(1):21-5. https://doi.org/10.1016/j.bbrc.2004.06.102

Author

Lützen, Anne ; Liberti, Sascha Emilie ; Rasmussen, Lene Juel. / Cadmium inhibits human DNA mismatch repair in vivo. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 321, No. 1. pp. 21-5.

Bibtex

@article{b1e345ed58fc46f3a5a0e4657089245d,
title = "Cadmium inhibits human DNA mismatch repair in vivo",
abstract = "The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repair activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reversed by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMR activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutS alpha and hEXO1-hMutL alpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair activity known to play an important role in colon cancer and that this effect can be reversed by Zn treatment.",
keywords = "Alkylation, Base Pair Mismatch, Base Sequence, Cadmium Chloride, Carcinogens, Cations, Divalent, Cell Cycle, Cell Line, DNA, DNA Damage, DNA Repair, DNA Repair Enzymes, Deoxyribonucleases, Exodeoxyribonucleases, Humans, Kidney, Kinetics, Oligodeoxyribonucleotides",
author = "Anne L{\"u}tzen and Liberti, {Sascha Emilie} and Rasmussen, {Lene Juel}",
year = "2004",
month = "8",
day = "13",
doi = "10.1016/j.bbrc.2004.06.102",
language = "English",
volume = "321",
pages = "21--5",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Cadmium inhibits human DNA mismatch repair in vivo

AU - Lützen, Anne

AU - Liberti, Sascha Emilie

AU - Rasmussen, Lene Juel

PY - 2004/8/13

Y1 - 2004/8/13

N2 - The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repair activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reversed by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMR activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutS alpha and hEXO1-hMutL alpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair activity known to play an important role in colon cancer and that this effect can be reversed by Zn treatment.

AB - The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repair activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reversed by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMR activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutS alpha and hEXO1-hMutL alpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair activity known to play an important role in colon cancer and that this effect can be reversed by Zn treatment.

KW - Alkylation

KW - Base Pair Mismatch

KW - Base Sequence

KW - Cadmium Chloride

KW - Carcinogens

KW - Cations, Divalent

KW - Cell Cycle

KW - Cell Line

KW - DNA

KW - DNA Damage

KW - DNA Repair

KW - DNA Repair Enzymes

KW - Deoxyribonucleases

KW - Exodeoxyribonucleases

KW - Humans

KW - Kidney

KW - Kinetics

KW - Oligodeoxyribonucleotides

U2 - 10.1016/j.bbrc.2004.06.102

DO - 10.1016/j.bbrc.2004.06.102

M3 - Journal article

C2 - 15358209

VL - 321

SP - 21

EP - 25

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

ID: 119639148