Cadmium inhibits human DNA mismatch repair in vivo
Research output: Contribution to journal › Journal article › Research › peer-review
The heavy metal cadmium (Cd) is a human carcinogen that inhibits DNA repair activities. We show that DNA mismatch repair (MMR)-mediated cell cycle arrest after alkylation damage is suppressed by exposure to Cd and that this effect is reversed by preincubation with excess of zinc (Zn). We show that Cd-mediated inactivation of MMR activity is not caused by disruption of complex formation between the MMR proteins hEXO1-hMutS alpha and hEXO1-hMutL alpha nor does Cd inhibit 5'-exonuclease activity of hEXO1 in vitro. Thus, our studies show that exposure of human cells to Cd suppresses MMR activity, a repair activity known to play an important role in colon cancer and that this effect can be reversed by Zn treatment.
|Journal||Biochemical and Biophysical Research Communications|
|Number of pages||5|
|Publication status||Published - 13 Aug 2004|
- Alkylation, Base Pair Mismatch, Base Sequence, Cadmium Chloride, Carcinogens, Cations, Divalent, Cell Cycle, Cell Line, DNA, DNA Damage, DNA Repair, DNA Repair Enzymes, Deoxyribonucleases, Exodeoxyribonucleases, Humans, Kidney, Kinetics, Oligodeoxyribonucleotides