Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Research output: Contribution to journalJournal articleResearchpeer-review

Branden M Hopkinson, Claus Desler Madsen, Mark Kalisz, Peter Siig Vestentoft, Lene Juel Rasmussen, Hanne Cathrine Bisgaard

Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.

Original languageEnglish
Article number5080128
JournalOxidative Medicine and Cellular Longevity
Volume2017
Number of pages11
ISSN1942-0900
DOIs
Publication statusPublished - 2017

    Research areas

  • Cell Differentiation, Cell Line, Cell Respiration, Energy Metabolism, Fluorescent Antibody Technique, Indirect, Hepatocytes, Human Embryonic Stem Cells, Humans, Microscopy, Confocal, Mitochondria, Polymerase Chain Reaction, Journal Article

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