Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Research output: Contribution to journal › Journal article › Research › peer-review

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Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage. / Hopkinson, Branden M; Madsen, Claus Desler; Kalisz, Mark; Vestentoft, Peter Siig; Juel Rasmussen, Lene; Bisgaard, Hanne Cathrine.

In: Oxidative Medicine and Cellular Longevity, Vol. 2017, 5080128, 2017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hopkinson, BM, Madsen, CD, Kalisz, M, Vestentoft, PS, Juel Rasmussen, L & Bisgaard, HC 2017, 'Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage', Oxidative Medicine and Cellular Longevity, vol. 2017, 5080128. https://doi.org/10.1155/2017/5080128

APA

Hopkinson, B. M., Madsen, C. D., Kalisz, M., Vestentoft, P. S., Juel Rasmussen, L., & Bisgaard, H. C. (2017). Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage. Oxidative Medicine and Cellular Longevity, 2017, [5080128]. https://doi.org/10.1155/2017/5080128

Vancouver

Hopkinson BM, Madsen CD, Kalisz M, Vestentoft PS, Juel Rasmussen L, Bisgaard HC. Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage. Oxidative Medicine and Cellular Longevity. 2017;2017. 5080128. https://doi.org/10.1155/2017/5080128

Author

Hopkinson, Branden M ; Madsen, Claus Desler ; Kalisz, Mark ; Vestentoft, Peter Siig ; Juel Rasmussen, Lene ; Bisgaard, Hanne Cathrine. / Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage. In: Oxidative Medicine and Cellular Longevity. 2017 ; Vol. 2017.

Bibtex

@article{3ee96786e975411ba6f407c5169141b5,
title = "Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage",
abstract = "Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.",
keywords = "Cell Differentiation, Cell Line, Cell Respiration, Energy Metabolism, Fluorescent Antibody Technique, Indirect, Hepatocytes, Human Embryonic Stem Cells, Humans, Microscopy, Confocal, Mitochondria, Polymerase Chain Reaction, Journal Article",
author = "Hopkinson, {Branden M} and Madsen, {Claus Desler} and Mark Kalisz and Vestentoft, {Peter Siig} and {Juel Rasmussen}, Lene and Bisgaard, {Hanne Cathrine}",
year = "2017",
doi = "10.1155/2017/5080128",
language = "English",
volume = "2017",
journal = "Oxidative Medicine and Cellular Longevity",
issn = "1942-0900",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

AU - Hopkinson, Branden M

AU - Madsen, Claus Desler

AU - Kalisz, Mark

AU - Vestentoft, Peter Siig

AU - Juel Rasmussen, Lene

AU - Bisgaard, Hanne Cathrine

PY - 2017

Y1 - 2017

N2 - Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.

AB - Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.

KW - Cell Differentiation

KW - Cell Line

KW - Cell Respiration

KW - Energy Metabolism

KW - Fluorescent Antibody Technique, Indirect

KW - Hepatocytes

KW - Human Embryonic Stem Cells

KW - Humans

KW - Microscopy, Confocal

KW - Mitochondria

KW - Polymerase Chain Reaction

KW - Journal Article

U2 - 10.1155/2017/5080128

DO - 10.1155/2017/5080128

M3 - Journal article

C2 - 28265337

VL - 2017

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

SN - 1942-0900

M1 - 5080128

ER -

ID: 179439798