Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

Research output: Contribution to journalJournal articleResearchpeer-review

There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

Original languageEnglish
JournalMethods in Molecular Biology
Pages (from-to)261-277
Number of pages17
Publication statusPublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

    Research areas

  • Caco-2, Cell viability, Cell-penetrating peptides as carriers, High-throughput screening, In vitro Mucus, Metabolic degradation, Permeability, Transepithelial drug delivery

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