Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro
Research output: Contribution to journal › Journal article › Research › peer-review
There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.
|Journal||Methods in Molecular Biology|
|Number of pages||17|
|Publication status||Published - 2015|
© Springer Science+Business Media New York 2015.
- Caco-2, Cell viability, Cell-penetrating peptides as carriers, High-throughput screening, In vitro Mucus, Metabolic degradation, Permeability, Transepithelial drug delivery