The LipB protein is a negative regulator of dam gene expression in Escherichia coli
Research output: Contribution to journal › Journal article › Research › peer-review
Transcription initiation of the major promoter (P2) of the Escherichia coli dam gene increases with growth rate. The presence of three partially palindromic motifs, (TTCAGT(N(20))TGAG), designated G (growth)-boxes, within the -52 to +31 region of the promoter, may be related to growth rate control. Deletion of two of these repeats, downstream of the transcription initiation point, result in constitutive high activity of the promoter. The unlinked cde-4::miniTn10 insertion also results in severalfold higher activity of the dam P2 promoter, suggesting that this mutation resulted in the loss of a putative dam P2 repressor. The cde-4 mutation was mapped to the lipB (lipoic acid) gene, which we show encodes a 24 kDa protein initiating at a TTG codon. LipB is a highly conserved protein in animal and plant species, other bacteria, Archaea, and yeast. Plasmids expressing the native or hexahistidine-tagged LipB complement the phenotype of the cde-4 mutant strain. The level of LipB in vivo was higher in exponentially growing cells than those in the stationary phase. Three G-box motifs were also found in the lipB region. Models for the regulation of expression of the two genes are discussed.
|Journal||Biochimica et Biophysica Acta, Gene Structure and Expression|
|Number of pages||11|
|Publication status||Published - 2000|
- Amino Acid Sequence, Bacterial Proteins/chemistry, Base Sequence, Blotting, Western, Cell Division, Codon, Initiator/genetics, Escherichia coli/genetics, Gene Expression Regulation, Bacterial, Genetic Complementation Test, Molecular Sequence Data, Mutation/genetics, Operon/genetics, Promoter Regions, Genetic/genetics, Recombinant Fusion Proteins/isolation & purification, Response Elements/genetics, Sequence Alignment