The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.

Original languageEnglish
Title of host publicationAntimicrobial Peptides
Number of pages9
PublisherHumana Press
Publication date2017
ISBN (Print)978-1-4939-6735-3
ISBN (Electronic)978-1-4939-6737-7
Publication statusPublished - 2017
SeriesMethods in Molecular Biology

    Research areas

  • Antimicrobial peptides, ATP leakage, DNA binding, Membrane perturbation, Mode of action, SOS response

ID: 188416444