Production of anti-PfEMP1 polyclonal antisera in rats and mice

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Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.

Original languageEnglish
Title of host publicationMalaria Immunology : Targeting the Surface of Infected Erythrocytes
EditorsAnja Tatiana Ramstedt Jensen, Lars Hviid
Number of pages9
PublisherHumana Press
Publication date2022
ISBN (Electronic)978-1-0716-2189-9
Publication statusPublished - 2022
SeriesMethods in molecular biology (Clifton, N.J.)

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Research areas

  • Animals, Antibodies, Protozoan, Endothelial Cells/metabolism, Erythrocytes/metabolism, Humans, Immune Sera/metabolism, Malaria, Falciparum, Mice, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism, Rats

ID: 327466186