Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

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Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. / Eriksen, Helle Brander; Petersen, Andreas; Pedersen, Michael; Overballe-Petersen, Søren; Larsen, Anders Rhod; Holzknecht, Barbara Juliane.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 41, No. 1, 2022, p. 163-167.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Eriksen, HB, Petersen, A, Pedersen, M, Overballe-Petersen, S, Larsen, AR & Holzknecht, BJ 2022, 'Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene', European Journal of Clinical Microbiology and Infectious Diseases, vol. 41, no. 1, pp. 163-167. https://doi.org/10.1007/s10096-021-04344-w

APA

Eriksen, H. B., Petersen, A., Pedersen, M., Overballe-Petersen, S., Larsen, A. R., & Holzknecht, B. J. (2022). Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. European Journal of Clinical Microbiology and Infectious Diseases, 41(1), 163-167. https://doi.org/10.1007/s10096-021-04344-w

Vancouver

Eriksen HB, Petersen A, Pedersen M, Overballe-Petersen S, Larsen AR, Holzknecht BJ. Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. European Journal of Clinical Microbiology and Infectious Diseases. 2022;41(1):163-167. https://doi.org/10.1007/s10096-021-04344-w

Author

Eriksen, Helle Brander ; Petersen, Andreas ; Pedersen, Michael ; Overballe-Petersen, Søren ; Larsen, Anders Rhod ; Holzknecht, Barbara Juliane. / Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. In: European Journal of Clinical Microbiology and Infectious Diseases. 2022 ; Vol. 41, No. 1. pp. 163-167.

Bibtex

@article{4489e540e2604707953b49eb7584e7c6,

title = "Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene",

abstract = "We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcusaureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.",

keywords = "blaZ, In vitro susceptibility testing, PSSA PRSA, Staphylococcus aureus",

author = "Eriksen, {Helle Brander} and Andreas Petersen and Michael Pedersen and S{\o}ren Overballe-Petersen and Larsen, {Anders Rhod} and Holzknecht, {Barbara Juliane}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2022",

doi = "10.1007/s10096-021-04344-w",

language = "English",

volume = "41",

pages = "163--167",

journal = "European Journal of Clinical Microbiology & Infectious Diseases",

issn = "0934-9723",

publisher = "Springer",

number = "1",

}

RIS

TY - JOUR

T1 - Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

AU - Eriksen, Helle Brander

AU - Petersen, Andreas

AU - Pedersen, Michael

AU - Overballe-Petersen, Søren

AU - Larsen, Anders Rhod

AU - Holzknecht, Barbara Juliane

PY - 2022

Y1 - 2022

N2 - We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcusaureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

AB - We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcusaureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

KW - blaZ

KW - In vitro susceptibility testing

KW - PSSA PRSA

KW - Staphylococcus aureus

U2 - 10.1007/s10096-021-04344-w

DO - 10.1007/s10096-021-04344-w

M3 - Journal article

C2 - 34529167

AN - SCOPUS:85115057061

VL - 41

SP - 163

EP - 167

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

SN - 0934-9723

IS - 1

ER -

ID: 313705552