New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder

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Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).

Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.

Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.

Original languageEnglish
Article number2001049
JournalMolecular Nutrition & Food Research
Issue number7
Number of pages9
Publication statusPublished - 2021

Bibliographical note

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    Research areas

  • Faculty of Science - Skimmed milk powder, Metabolomics, Advanced glycation end products, Maillard reaction, Biomarker

ID: 256631368