Cristian De Gobba
Preventive and Clinical Nutrition
Nørre Allé 51, 2200 København N.
Scopus Author ID: 55776253000
At this moment, my main research projects consist in the development of methods for the extraction, identification and quantification of different alcohol metabolites in hair, and in the development of a method for quantification of acetaldehyde adducts in haemoglobin, mainly using LC-MS. Furthermore, i continue to collaborate with my colleagues at the FOOD Department, where i my task is to extract and quantify dicarbonyls and advanced glycation end-products (AGEs) from food systems.
I also collaborated in different projects to establish LC-MS methods for the determination and quantification of small molecules such as Maillard reaction products and other oxidation products and characterized the peptide profile of milk protein hydrolysates and colostrum. Furthermore, I developed, together with other colleagues, a new LC-MS method for simultaneous determination of 12 different AGEs, and I established a method for quantification of thiol quinones adducts.
During my postdoc I gained extensive experience with protein and peptides identification using LC-MS: I worked, together with Danish Crown Ingredients, on production and characterisation of different blood hydrolysates for production of edible protein powders. These were analysed using LC-MS/MS, SEC-HPLC and UV-Vis spectroscopy. During my PhD project, I hydrolysed milk proteins with different enzymes and identified the resulting bioactive peptides using SEC-HPLC and RP-HPLC for separation and LC-MS for characterisation.