Neuropeptide y promotes neurogenesis in murine subventricular zone

Research output: Contribution to journalJournal articlepeer-review

  • Fabienne Agasse
  • Liliana Bernardino
  • Heidi Kristiansen
  • Søren H Christiansen
  • Raquel Ferreira
  • Bruno Silva
  • Sofia Grade
  • Woldbye, David Paul Drucker
  • João O Malva
  • Heidi Kristiansen
Stem cells of the subventricular zone (SVZ) represent a reliable source of neurons for cell replacement. Neuropeptide Y (NPY) promotes neurogenesis in the hippocampal subgranular layer and the olfactory epithelium and may be useful for the stimulation of SVZ dynamic in brain repair purposes. We describe that NPY promotes SVZ neurogenesis. NPY (1 microM) treatments increased proliferation at 48 hours and neuronal differentiation at 7 days in SVZ cell cultures. NPY proneurogenic properties are mediated via the Y1 receptor. Accordingly, Y1 receptor is a major active NPY receptor in the mouse SVZ, as shown by functional autoradiography. Moreover, short exposure to NPY increased immunoreactivity for the phosphorylated form of extracellular signal-regulated kinase 1/2 in the nucleus, compatible with a trigger for proliferation, whereas 6 hours of treatment amplified the phosphorylated form of c-Jun-NH(2)-terminal kinase signal in growing axons, consistent with axonogenesis. NPY, as a promoter of SVZ neurogenesis, is a crucial factor for future development of cell-based brain therapy. Disclosure of potential conflicts of interest is found at the end of this article.
Original languageEnglish
JournalStem Cells
Issue number6
Pages (from-to)1636-45
Number of pages9
Publication statusPublished - 2008

ID: 5161071