Neuropeptide y promotes neurogenesis in murine subventricular zone
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Neuropeptide y promotes neurogenesis in murine subventricular zone. / Agasse, Fabienne; Bernardino, Liliana; Kristiansen, Heidi; Christiansen, Søren H; Ferreira, Raquel; Silva, Bruno; Grade, Sofia; Woldbye, David P D; Malva, João O; Kristiansen, Heidi.
In: Stem Cells, Vol. 26, No. 6, 2008, p. 1636-45.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Neuropeptide y promotes neurogenesis in murine subventricular zone
AU - Agasse, Fabienne
AU - Bernardino, Liliana
AU - Kristiansen, Heidi
AU - Christiansen, Søren H
AU - Ferreira, Raquel
AU - Silva, Bruno
AU - Grade, Sofia
AU - Woldbye, David P D
AU - Malva, João O
AU - Kristiansen, Heidi
PY - 2008
Y1 - 2008
N2 - Stem cells of the subventricular zone (SVZ) represent a reliable source of neurons for cell replacement. Neuropeptide Y (NPY) promotes neurogenesis in the hippocampal subgranular layer and the olfactory epithelium and may be useful for the stimulation of SVZ dynamic in brain repair purposes. We describe that NPY promotes SVZ neurogenesis. NPY (1 microM) treatments increased proliferation at 48 hours and neuronal differentiation at 7 days in SVZ cell cultures. NPY proneurogenic properties are mediated via the Y1 receptor. Accordingly, Y1 receptor is a major active NPY receptor in the mouse SVZ, as shown by functional autoradiography. Moreover, short exposure to NPY increased immunoreactivity for the phosphorylated form of extracellular signal-regulated kinase 1/2 in the nucleus, compatible with a trigger for proliferation, whereas 6 hours of treatment amplified the phosphorylated form of c-Jun-NH(2)-terminal kinase signal in growing axons, consistent with axonogenesis. NPY, as a promoter of SVZ neurogenesis, is a crucial factor for future development of cell-based brain therapy. Disclosure of potential conflicts of interest is found at the end of this article.
AB - Stem cells of the subventricular zone (SVZ) represent a reliable source of neurons for cell replacement. Neuropeptide Y (NPY) promotes neurogenesis in the hippocampal subgranular layer and the olfactory epithelium and may be useful for the stimulation of SVZ dynamic in brain repair purposes. We describe that NPY promotes SVZ neurogenesis. NPY (1 microM) treatments increased proliferation at 48 hours and neuronal differentiation at 7 days in SVZ cell cultures. NPY proneurogenic properties are mediated via the Y1 receptor. Accordingly, Y1 receptor is a major active NPY receptor in the mouse SVZ, as shown by functional autoradiography. Moreover, short exposure to NPY increased immunoreactivity for the phosphorylated form of extracellular signal-regulated kinase 1/2 in the nucleus, compatible with a trigger for proliferation, whereas 6 hours of treatment amplified the phosphorylated form of c-Jun-NH(2)-terminal kinase signal in growing axons, consistent with axonogenesis. NPY, as a promoter of SVZ neurogenesis, is a crucial factor for future development of cell-based brain therapy. Disclosure of potential conflicts of interest is found at the end of this article.
U2 - 10.1634/stemcells.2008-0056
DO - 10.1634/stemcells.2008-0056
M3 - Journal article
C2 - 18388302
VL - 26
SP - 1636
EP - 1645
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 6
ER -
ID: 5161071