Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors
Research output: Contribution to journal › Journal article › Research › peer-review
The anorectic lipid oleoylethanolamide and the orexigenic lipid anandamide both belong to the group of N-acylethanolamines that are generated by the enzyme N-acylphosphatidylethanolamine-hydrolyzing phospholipase D. The levels of the two bioactive lipids were investigated in rat intestines after 24 h of starvation as well as after 1 and 4 h of re-feeding. Total levels of precursor phospholipids and N-acylethanolamines were decreased upon food-deprivation whereas the level of the anandamide precursor molecule was significantly increased. The level of 2-arachidonoyl-glycerol was unchanged as was the activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolyzing phospholipase D, and fatty acid amide hydrolase upon starvation and re-feeding. It is concluded that remodeling of the amide-linked fatty acids of N-acylphosphatidylethanolamine is responsible for the opposite effects on levels of anandamide and oleoylethanolamide in intestines of food-deprived rats and not an alternative biochemical route for anandamide synthesis. Furthermore, linoleoylethanolamide, which accounted for more than 50 mol% of the endogenous pool of N-acylethanolamines, was found not to have the same inhibitory effect on food intake, as did oleoylethanolamide following oral administration.
|BBA General Subjects
|143-50; discussion 141-2
|Published - Feb 2006
- Animals, Arachidonic Acids, Eating, Endocannabinoids, Food Deprivation, Intestines, Male, Oleic Acids, Phospholipids, Polyunsaturated Alkamides, Rats, Rats, Sprague-Dawley
- Former Faculty of Pharmaceutical Sciences