Immune monitoring using mRNA-transfected dendritic cells
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Dendritic cells are known to be the most potent antigen presenting cell in the immune system and are used as cellular adjuvants in therapeutic anticancer vaccines using various tumor-associated antigens or their derivatives. One way of loading antigen into the dendritic cells is by mRNA electroporation, ensuring presentation of antigen through major histocompatibility complex I and potentially activating T cells, enabling them to kill the tumor cells. Despite extensive research in the field, only one dendritic cell-based vaccine has been approved. There is therefore a great need to elucidate and understand the immunological impact of dendritic cell vaccination in order to improve clinical benefit. In this chapter, we describe a method for performing immune monitoring using peripheral blood mononuclear cells and autologous dendritic cells transfected with tumor-associated antigen-encoding mRNA.
Original language | English |
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Title of host publication | Synthetic mRNA : Production, Introduction Into Cells, and Physiological Consequences |
Number of pages | 15 |
Volume | 1428 |
Publisher | Humana Press |
Publication date | 2016 |
Pages | 245-259 |
ISBN (Print) | 978-1-4939-3623-6 |
ISBN (Electronic) | 978-1-4939-3625-0 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1428 |
ISSN | 1064-3745 |
- Cancer immunotherapy, Dendritic cell, Electroporation, Immune monitoring, mRNA transfection, Vaccination
Research areas
ID: 176375911