Full-length genomic analysis of Korean porcine Sapelovirus strains

Research output: Contribution to journalJournal articleResearchpeer-review

Porcine sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3' poly(A) tail, and showed the typical picornavirus genome organization; 5'untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3'UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5'UTR, a cis-replication element (CRE) in the 2C coding region and 3'UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3'UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.

Original languageEnglish
JournalPLoS ONE
Issue number9
Pages (from-to)e107860
Publication statusPublished - 2014
Externally publishedYes

    Research areas

  • 3' Untranslated Regions/genetics, 5' Untranslated Regions/genetics, Animals, Base Sequence, Genome, Viral/genetics, Genomics, Molecular Sequence Data, Phylogeny, Picornaviridae/genetics, Swine/virology

ID: 257915711