Full-length genomic analysis of Korean porcine Sapelovirus strains
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Full-length genomic analysis of Korean porcine Sapelovirus strains. / Son, Kyu-Yeol; Kim, Deok-Song; Kwon, Joseph; Choi, Jong-Soon; Kang, Mun-Il; Belsham, Graham J; Cho, Kyoung-Oh.
In: PLoS ONE, Vol. 9, No. 9, 2014, p. e107860.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Full-length genomic analysis of Korean porcine Sapelovirus strains
AU - Son, Kyu-Yeol
AU - Kim, Deok-Song
AU - Kwon, Joseph
AU - Choi, Jong-Soon
AU - Kang, Mun-Il
AU - Belsham, Graham J
AU - Cho, Kyoung-Oh
PY - 2014
Y1 - 2014
N2 - Porcine sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3' poly(A) tail, and showed the typical picornavirus genome organization; 5'untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3'UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5'UTR, a cis-replication element (CRE) in the 2C coding region and 3'UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3'UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.
AB - Porcine sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3' poly(A) tail, and showed the typical picornavirus genome organization; 5'untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3'UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5'UTR, a cis-replication element (CRE) in the 2C coding region and 3'UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3'UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.
KW - 3' Untranslated Regions/genetics
KW - 5' Untranslated Regions/genetics
KW - Animals
KW - Base Sequence
KW - Genome, Viral/genetics
KW - Genomics
KW - Molecular Sequence Data
KW - Phylogeny
KW - Picornaviridae/genetics
KW - Swine/virology
U2 - 10.1371/journal.pone.0107860
DO - 10.1371/journal.pone.0107860
M3 - Journal article
C2 - 25229940
VL - 9
SP - e107860
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 9
ER -
ID: 257915711