Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions
Research output: Contribution to journal › Journal article › peer-review
A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.
Original language | English |
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Journal | European Journal of Pharmaceutical Sciences |
Volume | 46 |
Issue number | 4 |
Pages (from-to) | 222-32 |
Number of pages | 11 |
ISSN | 0928-0987 |
DOIs | |
Publication status | Published - 2012 |
ID: 38229193