Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions. / Olesen, Niels Erik; Hofgaard, Johannes P; von Holstein-Rathlou, Niels-Henrik; Nielsen, Morten Schak; Jacobsen, Jens Christian Brings.

In: European Journal of Pharmaceutical Sciences, Vol. 46, No. 4, 2012, p. 222-32.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olesen, NE, Hofgaard, JP, von Holstein-Rathlou, N-H, Nielsen, MS & Jacobsen, JCB 2012, 'Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions', European Journal of Pharmaceutical Sciences, vol. 46, no. 4, pp. 222-32. https://doi.org/10.1016/j.ejps.2011.08.015

APA

Olesen, N. E., Hofgaard, J. P., von Holstein-Rathlou, N-H., Nielsen, M. S., & Jacobsen, J. C. B. (2012). Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions. European Journal of Pharmaceutical Sciences, 46(4), 222-32. https://doi.org/10.1016/j.ejps.2011.08.015

Vancouver

Olesen NE, Hofgaard JP, von Holstein-Rathlou N-H, Nielsen MS, Jacobsen JCB. Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions. European Journal of Pharmaceutical Sciences. 2012;46(4):222-32. https://doi.org/10.1016/j.ejps.2011.08.015

Author

Olesen, Niels Erik ; Hofgaard, Johannes P ; von Holstein-Rathlou, Niels-Henrik ; Nielsen, Morten Schak ; Jacobsen, Jens Christian Brings. / Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions. In: European Journal of Pharmaceutical Sciences. 2012 ; Vol. 46, No. 4. pp. 222-32.

Bibtex

@article{7898c3ac51464be6987611f65c713a74,

title = "Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions",

abstract = "A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.",

author = "Olesen, {Niels Erik} and Hofgaard, {Johannes P} and {von Holstein-Rathlou}, Niels-Henrik and Nielsen, {Morten Schak} and Jacobsen, {Jens Christian Brings}",

year = "2012",

doi = "10.1016/j.ejps.2011.08.015",

language = "English",

volume = "46",

pages = "222--32",

journal = "Norvegica Pharmaceutica Acta",

issn = "0928-0987",

publisher = "Elsevier",

number = "4",

}

RIS

TY - JOUR

T1 - Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions

AU - Olesen, Niels Erik

AU - Hofgaard, Johannes P

AU - von Holstein-Rathlou, Niels-Henrik

AU - Nielsen, Morten Schak

AU - Jacobsen, Jens Christian Brings

PY - 2012

Y1 - 2012

N2 - A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.

AB - A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.

U2 - 10.1016/j.ejps.2011.08.015

DO - 10.1016/j.ejps.2011.08.015

M3 - Journal article

C2 - 21888966

VL - 46

SP - 222

EP - 232

JO - Norvegica Pharmaceutica Acta

JF - Norvegica Pharmaceutica Acta

SN - 0928-0987

IS - 4

ER -

ID: 38229193