Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning

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This protocol describes how to order and directly assemble uracil-containing non-clonal DNA
fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of
making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is
highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from
any synthesizing company. The protocol drastically reduces time and handling between receiving the
synthesized DNA fragments and transforming with vector and DNA fragment(s).
Original languageEnglish
Article numbere2615
Issue number22
Number of pages11
Publication statusPublished - 2017

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