Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning. / Jørgensen, Morten Egevang; Wulff, Nikolai; Nafisi, Majse; Xu, Deyang; Wang, Cuiwei; Lambertz, Sophie Konstanze; Belew, Zeinu Mussa; Nour-Eldin, Hussam Hassan.

In: Bio-protocol, Vol. 7, No. 22, e2615, 2017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, ME, Wulff, N, Nafisi, M, Xu, D, Wang, C, Lambertz, SK, Belew, ZM & Nour-Eldin, HH 2017, 'Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning', Bio-protocol, vol. 7, no. 22, e2615. https://doi.org/10.21769/BioProtoc.2615

APA

Jørgensen, M. E., Wulff, N., Nafisi, M., Xu, D., Wang, C., Lambertz, S. K., Belew, Z. M., & Nour-Eldin, H. H. (2017). Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning. Bio-protocol, 7(22), [e2615]. https://doi.org/10.21769/BioProtoc.2615

Vancouver

Jørgensen ME, Wulff N, Nafisi M, Xu D, Wang C, Lambertz SK et al. Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning. Bio-protocol. 2017;7(22). e2615. https://doi.org/10.21769/BioProtoc.2615

Author

Jørgensen, Morten Egevang ; Wulff, Nikolai ; Nafisi, Majse ; Xu, Deyang ; Wang, Cuiwei ; Lambertz, Sophie Konstanze ; Belew, Zeinu Mussa ; Nour-Eldin, Hussam Hassan. / Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning. In: Bio-protocol. 2017 ; Vol. 7, No. 22.

Bibtex

@article{75932a0f195a4c74af71df402bc824b5,
title = "Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning",
abstract = "This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).",
author = "J{\o}rgensen, {Morten Egevang} and Nikolai Wulff and Majse Nafisi and Deyang Xu and Cuiwei Wang and Lambertz, {Sophie Konstanze} and Belew, {Zeinu Mussa} and Nour-Eldin, {Hussam Hassan}",
year = "2017",
doi = "10.21769/BioProtoc.2615",
language = "English",
volume = "7",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "22",

}

RIS

TY - JOUR

T1 - Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning

AU - Jørgensen, Morten Egevang

AU - Wulff, Nikolai

AU - Nafisi, Majse

AU - Xu, Deyang

AU - Wang, Cuiwei

AU - Lambertz, Sophie Konstanze

AU - Belew, Zeinu Mussa

AU - Nour-Eldin, Hussam Hassan

PY - 2017

Y1 - 2017

N2 - This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).

AB - This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).

U2 - 10.21769/BioProtoc.2615

DO - 10.21769/BioProtoc.2615

M3 - Journal article

C2 - 34595288

VL - 7

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 22

M1 - e2615

ER -

ID: 188708667