Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles
Research output: Contribution to journal › Journal article › Research › peer-review
A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.
Original language | English |
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Journal | Bioconjugate Chemistry |
Volume | 17 |
Issue number | 6 |
Pages (from-to) | 1373-1375 |
Number of pages | 3 |
ISSN | 1043-1802 |
DOIs | |
Publication status | Published - 2006 |
Externally published | Yes |
ID: 286412079