Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Bionanoconjugation via click chemistry : The creation of functional hybrids of lipases and gold nanoparticles. / Brennan, Jennifer L.; Hatzakis, Nikos S.; Tshikhudo, T. Robert; Dirvianskyte, Nijole; Razumas, Valdemaras; Patkar, Shamkant; Vind, Jesper; Svendsen, Allan; Nolte, Roeland J.M.; Rowan, Alan E.; Brust, Mathias.

In: Bioconjugate Chemistry, Vol. 17, No. 6, 2006, p. 1373-1375.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Brennan, JL, Hatzakis, NS, Tshikhudo, TR, Dirvianskyte, N, Razumas, V, Patkar, S, Vind, J, Svendsen, A, Nolte, RJM, Rowan, AE & Brust, M 2006, 'Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles', Bioconjugate Chemistry, vol. 17, no. 6, pp. 1373-1375. https://doi.org/10.1021/bc0601018

APA

Brennan, J. L., Hatzakis, N. S., Tshikhudo, T. R., Dirvianskyte, N., Razumas, V., Patkar, S., Vind, J., Svendsen, A., Nolte, R. J. M., Rowan, A. E., & Brust, M. (2006). Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles. Bioconjugate Chemistry, 17(6), 1373-1375. https://doi.org/10.1021/bc0601018

Vancouver

Brennan JL, Hatzakis NS, Tshikhudo TR, Dirvianskyte N, Razumas V, Patkar S et al. Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles. Bioconjugate Chemistry. 2006;17(6):1373-1375. https://doi.org/10.1021/bc0601018

Author

Brennan, Jennifer L. ; Hatzakis, Nikos S. ; Tshikhudo, T. Robert ; Dirvianskyte, Nijole ; Razumas, Valdemaras ; Patkar, Shamkant ; Vind, Jesper ; Svendsen, Allan ; Nolte, Roeland J.M. ; Rowan, Alan E. ; Brust, Mathias. / Bionanoconjugation via click chemistry : The creation of functional hybrids of lipases and gold nanoparticles. In: Bioconjugate Chemistry. 2006 ; Vol. 17, No. 6. pp. 1373-1375.

Bibtex

@article{aaa478bbf87e467ea6d2336a7544a7b0,
title = "Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles",
abstract = "A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using {"}click{"} chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.",
author = "Brennan, {Jennifer L.} and Hatzakis, {Nikos S.} and Tshikhudo, {T. Robert} and Nijole Dirvianskyte and Valdemaras Razumas and Shamkant Patkar and Jesper Vind and Allan Svendsen and Nolte, {Roeland J.M.} and Rowan, {Alan E.} and Mathias Brust",
year = "2006",
doi = "10.1021/bc0601018",
language = "English",
volume = "17",
pages = "1373--1375",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "6",

}

RIS

TY - JOUR

T1 - Bionanoconjugation via click chemistry

T2 - The creation of functional hybrids of lipases and gold nanoparticles

AU - Brennan, Jennifer L.

AU - Hatzakis, Nikos S.

AU - Tshikhudo, T. Robert

AU - Dirvianskyte, Nijole

AU - Razumas, Valdemaras

AU - Patkar, Shamkant

AU - Vind, Jesper

AU - Svendsen, Allan

AU - Nolte, Roeland J.M.

AU - Rowan, Alan E.

AU - Brust, Mathias

PY - 2006

Y1 - 2006

N2 - A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.

AB - A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.

U2 - 10.1021/bc0601018

DO - 10.1021/bc0601018

M3 - Journal article

C2 - 17105213

AN - SCOPUS:33845217955

VL - 17

SP - 1373

EP - 1375

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 6

ER -

ID: 286412079