Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis

Research output: Contribution to journalJournal articlepeer-review

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Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis. / Titze, Isabel; Krömker, Volker.

In: Veterinary Sciences, Vol. 7, No. 1, 31, 2020.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Titze, I & Krömker, V 2020, 'Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis', Veterinary Sciences, vol. 7, no. 1, 31. https://doi.org/10.3390/vetsci7010031

APA

Titze, I., & Krömker, V. (2020). Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis. Veterinary Sciences, 7(1), [31]. https://doi.org/10.3390/vetsci7010031

Vancouver

Titze I, Krömker V. Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis. Veterinary Sciences. 2020;7(1). 31. https://doi.org/10.3390/vetsci7010031

Author

Titze, Isabel ; Krömker, Volker. / Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis. In: Veterinary Sciences. 2020 ; Vol. 7, No. 1.

Bibtex

@article{a4835175e4404185bb7b545e75d7ff13,
title = "Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis",
abstract = "The antimicrobial activity of a phagemixture and a lactic acid bacteriumagainst Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1. ST29, EB1. ST11 and EB1. ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.",
keywords = "Bacteriophage mixture, Bovine mastitis, Dairy, Lactic acid bacterium, Lytic phage, Phage therapy, Staphylococcus aureus",
author = "Isabel Titze and Volker Kr{\"o}mker",
year = "2020",
doi = "10.3390/vetsci7010031",
language = "English",
volume = "7",
journal = "Veterinary Sciences",
issn = "2306-7381",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "1",

}

RIS

TY - JOUR

T1 - Antimicrobial activity of a phage mixture and a lactic acid bacterium against staphylococcus aureus from bovine mastitis

AU - Titze, Isabel

AU - Krömker, Volker

PY - 2020

Y1 - 2020

N2 - The antimicrobial activity of a phagemixture and a lactic acid bacteriumagainst Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1. ST29, EB1. ST11 and EB1. ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.

AB - The antimicrobial activity of a phagemixture and a lactic acid bacteriumagainst Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1. ST29, EB1. ST11 and EB1. ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.

KW - Bacteriophage mixture

KW - Bovine mastitis

KW - Dairy

KW - Lactic acid bacterium

KW - Lytic phage

KW - Phage therapy

KW - Staphylococcus aureus

U2 - 10.3390/vetsci7010031

DO - 10.3390/vetsci7010031

M3 - Journal article

C2 - 32155751

AN - SCOPUS:85081919379

VL - 7

JO - Veterinary Sciences

JF - Veterinary Sciences

SN - 2306-7381

IS - 1

M1 - 31

ER -

ID: 239014795