Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells

Research output: Contribution to journal › Journal article › Research › peer-review

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Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. / Adinolfi, Barbara; Carpi, Sara; Romanini, Antonella; Da Pozzo, Eleonora; Castagna, Maura; Costa, Barbara; Martini, Claudia; Olesen, Søren-Peter; Schmitt, Nicole; Breschi, Maria Cristina; Nieri, Paola; Fogli, Stefano.

In: Anticancer Research, Vol. 35, No. 7, 07.2015, p. 3781-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Adinolfi, B, Carpi, S, Romanini, A, Da Pozzo, E, Castagna, M, Costa, B, Martini, C, Olesen, S-P, Schmitt, N, Breschi, MC, Nieri, P & Fogli, S 2015, 'Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells', Anticancer Research, vol. 35, no. 7, pp. 3781-6. <http://ar.iiarjournals.org/content/35/7/3781.long>

APA

Adinolfi, B., Carpi, S., Romanini, A., Da Pozzo, E., Castagna, M., Costa, B., Martini, C., Olesen, S-P., Schmitt, N., Breschi, M. C., Nieri, P., & Fogli, S. (2015). Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. Anticancer Research, 35(7), 3781-6. http://ar.iiarjournals.org/content/35/7/3781.long

Vancouver

Adinolfi B, Carpi S, Romanini A, Da Pozzo E, Castagna M, Costa B et al. Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. Anticancer Research. 2015 Jul;35(7):3781-6.

Author

Adinolfi, Barbara ; Carpi, Sara ; Romanini, Antonella ; Da Pozzo, Eleonora ; Castagna, Maura ; Costa, Barbara ; Martini, Claudia ; Olesen, Søren-Peter ; Schmitt, Nicole ; Breschi, Maria Cristina ; Nieri, Paola ; Fogli, Stefano. / Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. In: Anticancer Research. 2015 ; Vol. 35, No. 7. pp. 3781-6.

Bibtex

@article{f3cb43923a5b4e6a96e7cca75268cc72,

title = "Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells",

abstract = "AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.",

keywords = "Annexin A5, Antineoplastic Agents, Apoptosis, Cell Line, Tumor, Cell Survival, Cisplatin, Clotrimazole, DNA Fragmentation, G1 Phase Cell Cycle Checkpoints, Humans, Keratinocytes, Melanoma",

author = "Barbara Adinolfi and Sara Carpi and Antonella Romanini and {Da Pozzo}, Eleonora and Maura Castagna and Barbara Costa and Claudia Martini and S{\o}ren-Peter Olesen and Nicole Schmitt and Breschi, {Maria Cristina} and Paola Nieri and Stefano Fogli",

year = "2015",

month = jul,

language = "English",

volume = "35",

pages = "3781--6",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "7",

}

RIS

TY - JOUR

T1 - Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells

AU - Adinolfi, Barbara

AU - Carpi, Sara

AU - Romanini, Antonella

AU - Da Pozzo, Eleonora

AU - Castagna, Maura

AU - Costa, Barbara

AU - Martini, Claudia

AU - Olesen, Søren-Peter

AU - Schmitt, Nicole

AU - Breschi, Maria Cristina

AU - Nieri, Paola

AU - Fogli, Stefano

PY - 2015/7

Y1 - 2015/7

N2 - AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.

AB - AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.

KW - Annexin A5

KW - Antineoplastic Agents

KW - Apoptosis

KW - Cell Line, Tumor

KW - Cell Survival

KW - Cisplatin

KW - Clotrimazole

KW - DNA Fragmentation

KW - G1 Phase Cell Cycle Checkpoints

KW - Humans

KW - Keratinocytes

KW - Melanoma

M3 - Journal article

C2 - 26124322

VL - 35

SP - 3781

EP - 3786

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 7

ER -

ID: 149044239