Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells

Research output: Contribution to journalJournal articleResearchpeer-review

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Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. / Adinolfi, Barbara; Carpi, Sara; Romanini, Antonella; Da Pozzo, Eleonora; Castagna, Maura; Costa, Barbara; Martini, Claudia; Olesen, Søren-Peter; Schmitt, Nicole; Breschi, Maria Cristina; Nieri, Paola; Fogli, Stefano.

In: Anticancer Research, Vol. 35, No. 7, 07.2015, p. 3781-6.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Adinolfi, B, Carpi, S, Romanini, A, Da Pozzo, E, Castagna, M, Costa, B, Martini, C, Olesen, S-P, Schmitt, N, Breschi, MC, Nieri, P & Fogli, S 2015, 'Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells', Anticancer Research, vol. 35, no. 7, pp. 3781-6.

APA

Adinolfi, B., Carpi, S., Romanini, A., Da Pozzo, E., Castagna, M., Costa, B., ... Fogli, S. (2015). Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. Anticancer Research, 35(7), 3781-6.

Vancouver

Adinolfi B, Carpi S, Romanini A, Da Pozzo E, Castagna M, Costa B et al. Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. Anticancer Research. 2015 Jul;35(7):3781-6.

Author

Adinolfi, Barbara ; Carpi, Sara ; Romanini, Antonella ; Da Pozzo, Eleonora ; Castagna, Maura ; Costa, Barbara ; Martini, Claudia ; Olesen, Søren-Peter ; Schmitt, Nicole ; Breschi, Maria Cristina ; Nieri, Paola ; Fogli, Stefano. / Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. In: Anticancer Research. 2015 ; Vol. 35, No. 7. pp. 3781-6.

Bibtex

@article{f3cb43923a5b4e6a96e7cca75268cc72,
title = "Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells",
abstract = "AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50{\%} (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.",
keywords = "Annexin A5, Antineoplastic Agents, Apoptosis, Cell Line, Tumor, Cell Survival, Cisplatin, Clotrimazole, DNA Fragmentation, G1 Phase Cell Cycle Checkpoints, Humans, Keratinocytes, Melanoma",
author = "Barbara Adinolfi and Sara Carpi and Antonella Romanini and {Da Pozzo}, Eleonora and Maura Castagna and Barbara Costa and Claudia Martini and S{\o}ren-Peter Olesen and Nicole Schmitt and Breschi, {Maria Cristina} and Paola Nieri and Stefano Fogli",
note = "Copyright{\circledC} 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.",
year = "2015",
month = "7",
language = "English",
volume = "35",
pages = "3781--6",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "7",

}

RIS

TY - JOUR

T1 - Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells

AU - Adinolfi, Barbara

AU - Carpi, Sara

AU - Romanini, Antonella

AU - Da Pozzo, Eleonora

AU - Castagna, Maura

AU - Costa, Barbara

AU - Martini, Claudia

AU - Olesen, Søren-Peter

AU - Schmitt, Nicole

AU - Breschi, Maria Cristina

AU - Nieri, Paola

AU - Fogli, Stefano

N1 - Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

PY - 2015/7

Y1 - 2015/7

N2 - AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.

AB - AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.

KW - Annexin A5

KW - Antineoplastic Agents

KW - Apoptosis

KW - Cell Line, Tumor

KW - Cell Survival

KW - Cisplatin

KW - Clotrimazole

KW - DNA Fragmentation

KW - G1 Phase Cell Cycle Checkpoints

KW - Humans

KW - Keratinocytes

KW - Melanoma

M3 - Journal article

C2 - 26124322

VL - 35

SP - 3781

EP - 3786

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 7

ER -

ID: 149044239