Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells

Research output: Contribution to journalJournal articleResearchpeer-review

  • Barbara Adinolfi
  • Sara Carpi
  • Antonella Romanini
  • Eleonora Da Pozzo
  • Maura Castagna
  • Barbara Costa
  • Claudia Martini
  • Olesen, Søren-Peter
  • Schmitt, Nicole
  • Maria Cristina Breschi
  • Paola Nieri
  • Stefano Fogli

AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.

MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.

RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.

CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.

Original languageEnglish
JournalAnticancer Research
Issue number7
Pages (from-to)3781-6
Number of pages6
Publication statusPublished - Jul 2015

    Research areas

  • Annexin A5, Antineoplastic Agents, Apoptosis, Cell Line, Tumor, Cell Survival, Cisplatin, Clotrimazole, DNA Fragmentation, G1 Phase Cell Cycle Checkpoints, Humans, Keratinocytes, Melanoma

ID: 149044239