Affinity purification of PfEMP1-specific antibodies from human blood

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Acquired immunity against Plasmodium falciparum infections relies heavily on IgG antibodies specific for PfEMP1 proteins expressed on the surface of infected erythrocytes. Purified human antibodies can be used, for example, to study the interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells, and to identify which IgG antibodies play a functional role in natural acquired immunity.This chapter describe how to affinity purify PfEMP1-specific human antibodies on an affinity column coupled with PfEMP1 protein. We include ELISA-based methods for identification of human plasma samples reactive against PfEMP1, and for testing of affinity purified IgG antibodies prior to their use in more advanced procedures.

Original languageEnglish
Title of host publicationMalaria Immunology : Targeting the Surface of Infected Erythrocytes
Number of pages11
PublisherHumana Press
Publication date2022
ISBN (Print)978-1-0716-2188-2
ISBN (Electronic)978-1-0716-2189-9
Publication statusPublished - 2022
SeriesMethods in molecular biology (Clifton, N.J.)

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Research areas

  • Antibodies, Protozoan, Antigens, Protozoan, Endothelial Cells/metabolism, Erythrocytes/metabolism, Humans, Immunoglobulin G, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism

ID: 317099436