3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.
Original language | English |
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Title of host publication | Malaria Immunology : Targeting the Surface of Infected Erythrocytes |
Number of pages | 13 |
Volume | 2470 |
Publisher | Humana Press |
Publication date | 2022 |
Pages | 587-599 |
ISBN (Electronic) | 978-1-0716-2189-9 |
DOIs | |
Publication status | Published - 2022 |
Series | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
Bibliographical note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Blood-Brain Barrier/metabolism, Cell Adhesion/physiology, Endothelial Cells/metabolism, Erythrocytes/metabolism, Humans, Malaria, Falciparum/parasitology, Organoids/metabolism, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism
Research areas
ID: 320648836