Chuna Ram Choudhary
Professor, Group Leader
Dr. Chuna Ram Choudhary is currently a professor and group leader at the Novo Nordisk Foundation Center for Protein Research at the University of Copenhagen in Denmark. He carried out his doctoral studies at the University of Münster in Germany (under Prof. Dr. Hubert Serve) and his postdoctoral training at the same university as well as at the Max Planck Institute for Biochemistry in Martinsried, Germany (under Prof. Dr. Matthias Mann). Dr. Choudhary has been widely published in many reputable, peer-reviewed journals and in 2012, was awarded a prestigious Sapere Aude research grant from the Danish Research Council. His laboratory is interested in investigating the dynamics of protein posttranslational modifications, in particular of lysine acetylation and ubiquitylation, in cell signaling networks using quantitative mass spectrometry-based approaches.
Primary fields of research
Biological systems respond to a wide range of extracellular and intracellular cues (signals), resulting in a myriad of diverse outcomes. Cells respond to these signals by modulating expression levels of protein as well as by regulating their functions via posttranslational modifications (PTMs). PTMs have critical roles in the dynamic regulation of protein activity, stability, spatial localization, and communication with other macromolecules within cells. Deregulation of PTM modifying enzymes, such as protein kinases, ubiquitin ligases, and lysine deacetylases, is often observed in human diseases further highlighting the importance of their regulatory functions. Thus, unraveling the scope of PTMs, their link to regulatory enzymes, and their dynamics in response to cellular perturbations is critically important for obtaining a better understanding of the principles of cellular governance.
A major focus of the Group is to obtain a 'systems-wide' understanding of signaling networks. For this, our laboratory applies cutting-edge mass spectrometry-based proteomics technologies for unbiased, global, and quantitative analysis of key regulatory PTMs. We work closely with other groups within the Centre as well as with our national and international collaborators.
Selected publications
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Functions and mechanisms of non-histone protein acetylation
Narita, Takeo, Weinert, B. T. & Choudhary, Chunaram, 2019, In: Nature Reviews. Molecular Cell Biology. 20, p. 156-174 19 p.Research output: Contribution to journal › Review › Research › peer-review
- Published
Analysis and Interpretation of Protein Post-Translational Modification Site Stoichiometry
Prus, Gabriela Zofia, Hoegl, A., Weinert, B. T. & Choudhary, Chunaram, 2019, In: Trends in Biochemical Sciences. 44, 11, p. 943-960Research output: Contribution to journal › Review › Research › peer-review
- Published
Time-Resolved Analysis Reveals Rapid Dynamics and Broad Scope of the CBP/p300 Acetylome
Weinert, B. T., Narita, Takeo, Satpathy, S., Srinivasan, B., Hansen, B. K., Schölz, C., Hamilton, W. B., Zucconi, B. E., Wang, W. W., Liu, W. R., Brickman, Joshua Mark, Kesicki, E. A., Lai, A., Bromberg, K. D., Cole, P. A. & Choudhary, Chunaram, 2018, In: Cell. 174, 1, p. 231-244.e12 27 p.Research output: Contribution to journal › Journal article › Research › peer-review
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DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity
Gupta, R., Somyajit, K., Narita, Takeo, Maskey, E., Stanlie, A., Kremer, M., Typas, D., Lammers, M., Mailand, Niels, Nussenzweig, A., Lukas, Jiri & Choudhary, Chunaram, 2018, In: Cell. 173, 4, p. 972-988Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Acetylation site specificities of lysine deacetylase inhibitors in human cells
Schölz, C., Weinert, B. T., Wagner, S. A., Beli, P., Miyake, Y., Qi, J., Jensen, Lars Juhl, Streicher, W., McCarthy, A. R., Westwood, N. J., Lain, S., Cox, J., Matthias, P., Mann, M., Bradner, J. E. & Choudhary, Chunaram, 2015, In: Nature Biotechnology. 33, p. 415–423Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The growing landscape of lysine acetylation links metabolism and cell signalling
Choudhary, Chunaram, Weinert, B. T., Nishida, Y., Verdin, E. & Mann, Matthias, 23 Jul 2014, In: Nature Reviews. Molecular Cell Biology. 15, 8, p. 536-50 15 p.Research output: Contribution to journal › Review › Research › peer-review
- Published
Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli
Weinert, B. T., Iesmantavicius, V., Wagner, S. A., Schölz, C., Gummesson, Bertil, Beli, P., Nyström, T. & Choudhary, Chunaram, 25 Jul 2013, In: Molecular Cell. 51, 2, p. 265-272 8 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass
Povlsen, L. K., Beli, P., Wagner, S. A., Poulsen, S. L., Sylvestersen, K. B., Poulsen, J. W., Nielsen, Michael Lund, Bekker-Jensen, Simon Holst, Mailand, Niels & Choudhary, Chunaram, Oct 2012, In: Nature Cell Biology. 14, 10, p. 1089-98 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response
Beli, P., Lukashchuk, N., Wagner, S. A., Weinert, B. T., Olsen, Jesper Velgaard, Baskcomb, L., Mann, Matthias, Jackson, S. P. & Choudhary, Chunaram, 2012, In: Molecular Cell. 46, 2, p. 212-25 14 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Lysine acetylation targets protein complexes and co-regulates major cellular functions
Choudhary, Chunaram, Kumar, C., Gnad, F., Nielsen, Michael Lund, Rehman, M., Walther, T. C., Olsen, Jesper Velgaard & Mann, Matthias, 2009, In: Science. 325, 5942, p. 834-40 6 p.Research output: Contribution to journal › Journal article › Research › peer-review
ID: 13781159
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Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage
Research output: Contribution to journal › Letter › Research › peer-review
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Deubiquitylating enzyme USP9x regulates hippo pathway activity by controlling angiomotin protein turnover
Research output: Contribution to journal › Journal article › Research › peer-review
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269
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Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
Research output: Contribution to journal › Journal article › Research › peer-review
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