Lysine acetylation targets protein complexes and co-regulates major cellular functions
Research output: Contribution to journal › Journal article › Research › peer-review
Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications.
Original language | English |
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Journal | Science |
Volume | 325 |
Issue number | 5942 |
Pages (from-to) | 834-40 |
Number of pages | 6 |
ISSN | 0036-8075 |
DOIs | |
Publication status | Published - 2009 |
Bibliographical note
Keywords: Acetylation; Amino Acid Motifs; Benzamides; Cell Line, Tumor; Cell Nucleus; Cell Physiological Phenomena; Cytoplasm; Enzyme Inhibitors; Histone Deacetylases; Humans; Hydroxamic Acids; Lysine; Mass Spectrometry; Metabolic Networks and Pathways; Mitochondria; Multiprotein Complexes; Protein Processing, Post-Translational; Protein Structure, Tertiary; Proteins; Proteome; Proteomics; Pyridines; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
ID: 14701277