Selecting Schizosaccharomyces pombe diploids
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Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.
Original language | English |
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Journal | Cold Spring Harbor Protocols |
Volume | 2017 |
Issue number | 7 |
Pages (from-to) | 537-540 |
Number of pages | 4 |
ISSN | 1940-3402 |
DOIs | |
Publication status | Published - Jul 2017 |
ID: 181456120