Selecting Schizosaccharomyces pombe diploids

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Selecting Schizosaccharomyces pombe diploids. / Ekwall, Karl; Thon, Genevieve.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 7, 07.2017, p. 537-540.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ekwall, K & Thon, G 2017, 'Selecting Schizosaccharomyces pombe diploids', Cold Spring Harbor Protocols, vol. 2017, no. 7, pp. 537-540. https://doi.org/10.1101/pdb.prot091702

APA

Ekwall, K., & Thon, G. (2017). Selecting Schizosaccharomyces pombe diploids. Cold Spring Harbor Protocols, 2017(7), 537-540. https://doi.org/10.1101/pdb.prot091702

Vancouver

Ekwall K, Thon G. Selecting Schizosaccharomyces pombe diploids. Cold Spring Harbor Protocols. 2017 Jul;2017(7):537-540. https://doi.org/10.1101/pdb.prot091702

Author

Ekwall, Karl ; Thon, Genevieve. / Selecting Schizosaccharomyces pombe diploids. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 7. pp. 537-540.

Bibtex

@article{72d5d7e5f5454f11b1f0ed7ee8d6307a,
title = "Selecting Schizosaccharomyces pombe diploids",
abstract = "Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.",
author = "Karl Ekwall and Genevieve Thon",
year = "2017",
month = jul,
doi = "10.1101/pdb.prot091702",
language = "English",
volume = "2017",
pages = "537--540",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
publisher = "Cold Spring Harbor Laboratory Press",
number = "7",

}

RIS

TY - JOUR

T1 - Selecting Schizosaccharomyces pombe diploids

AU - Ekwall, Karl

AU - Thon, Genevieve

PY - 2017/7

Y1 - 2017/7

N2 - Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.

AB - Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.

UR - http://www.scopus.com/inward/record.url?scp=85021729779&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot091702

DO - 10.1101/pdb.prot091702

M3 - Journal article

C2 - 28679702

AN - SCOPUS:85021729779

VL - 2017

SP - 537

EP - 540

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 7

ER -

ID: 181456120