Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags

Research output: Contribution to journalJournal articleResearchpeer-review


  • Fulltext

    Final published version, 537 KB, PDF document

  • Susanna Cirera
  • Jakob Hedegaard
  • Michael J Gilchrist
  • Frank Panitz
  • Karsten Scheibye-Knudsen
  • Troels Arvin
  • Steen Lumholdt
  • Milena Sawera
  • Trine Green
  • Bente Nielsen
  • Carina Rosenkilde
  • Jun Wang
  • Heng Li
  • Ruiqiang Li
  • Bin Liu
  • Songnian Hu
  • Wei Dong
  • Wei Li
  • Jun Yu
  • Jian Wang
  • Hans-Henrik Staefeldt
  • Rasmus Wernersson
  • Lone B Madsen
  • Bo Stjerne Thomsen
  • Henrik Hornshøj
  • Zhan Bujie
  • Xuegang Wang
  • Xuefei Wang
  • Lars Bolund
  • Huanming Yang
  • Christian Bendixen
Background: Knowledge of the structure of gene expression is essential for mammalian transcriptomics research. We analyzed a collection of more than one million porcine expressed sequence tags (ESTs), of which two-thirds were generated in the Sino-Danish Pig Genome Project and one-third are from public databases. The Sino-Danish ESTs were generated from one normalized and 97 non-normalized cDNA libraries representing 35 different tissues and three developmental stages.
Results: Using the Distiller package, the ESTs were assembled to roughly 48,000 contigs and 73,000 singletons, of which approximately 25% have a high confidence match to UniProt. Approximately 6,000 new porcine gene clusters were identified. Expression analysis based on the non-normalized libraries resulted in the following findings. The distribution of cluster sizes is scaling invariant. Brain and testes are among the tissues with the greatest number of different expressed genes, whereas tissues with more specialized function, such as developing liver, have fewer expressed genes. There are at least 65 high confidence housekeeping gene candidates and 876 cDNA library-specific gene candidates. We identified differential expression of genes between different tissues, in particular brain/spinal cord, and found patterns of correlation between genes that share expression in pairs of libraries. Finally, there was remarkable agreement in expression between specialized tissues according to Gene Ontology categories.
Conclusion: This EST collection, the largest to date in pig, represents an essential resource for annotation, comparative genomics, assembly of the pig genome sequence, and further porcine transcription studies.
Original languageEnglish
JournalGenome Biology
Issue numberR45
Pages (from-to)R45
Number of pages16
Publication statusPublished - 2007

    Research areas

  • Animals, Cluster Analysis, Computational Biology, Expressed Sequence Tags, Gene Expression, Gene Expression Profiling, Gene Library, Genomics, Multigene Family, RNA, Messenger, Swine

Number of downloads are based on statistics from Google Scholar and www.ku.dk

No data available

ID: 8067734