Peptide release, side-chain deprotection, work-up, and isolation

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Peptide release, side-chain deprotection, work-up, and isolation. / Pedersen, Søren Ljungberg; Jensen, Knud Jørgen.

Peptide synthesis and applications. ed. / Knud J. Jensen; Pernille T. Shelton; Søren L. Pedersen. Humana Press, 2013. p. 43-63 (Methods in Molecular Biology, Vol. 1047).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Pedersen, SL & Jensen, KJ 2013, Peptide release, side-chain deprotection, work-up, and isolation. in KJ Jensen, PT Shelton & SL Pedersen (eds), Peptide synthesis and applications. Humana Press, Methods in Molecular Biology, vol. 1047, pp. 43-63. https://doi.org/10.1007/978-1-62703-544-6_3

APA

Pedersen, S. L., & Jensen, K. J. (2013). Peptide release, side-chain deprotection, work-up, and isolation. In K. J. Jensen, P. T. Shelton, & S. L. Pedersen (Eds.), Peptide synthesis and applications (pp. 43-63). Humana Press. Methods in Molecular Biology Vol. 1047 https://doi.org/10.1007/978-1-62703-544-6_3

Vancouver

Pedersen SL, Jensen KJ. Peptide release, side-chain deprotection, work-up, and isolation. In Jensen KJ, Shelton PT, Pedersen SL, editors, Peptide synthesis and applications. Humana Press. 2013. p. 43-63. (Methods in Molecular Biology, Vol. 1047). https://doi.org/10.1007/978-1-62703-544-6_3

Author

Pedersen, Søren Ljungberg ; Jensen, Knud Jørgen. / Peptide release, side-chain deprotection, work-up, and isolation. Peptide synthesis and applications. editor / Knud J. Jensen ; Pernille T. Shelton ; Søren L. Pedersen. Humana Press, 2013. pp. 43-63 (Methods in Molecular Biology, Vol. 1047).

Bibtex

@inbook{c80a8cd9291a4f878a38641f4454ce50,
title = "Peptide release, side-chain deprotection, work-up, and isolation",
abstract = "After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide Purities and for storage conditions are provided.",
keywords = "Peptide cleavage, Side-chain deprotection, Rink amide linker, Wang linker, Hydrazine benzoyl linker, HMBA linker",
author = "Pedersen, {S{\o}ren Ljungberg} and Jensen, {Knud J{\o}rgen}",
year = "2013",
doi = "10.1007/978-1-62703-544-6_3",
language = "English",
isbn = "978-1-62703-543-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "43--63",
editor = "Jensen, {Knud J.} and Shelton, {Pernille T.} and Pedersen, {S{\o}ren L.}",
booktitle = "Peptide synthesis and applications",
address = "United States",

}

RIS

TY - CHAP

T1 - Peptide release, side-chain deprotection, work-up, and isolation

AU - Pedersen, Søren Ljungberg

AU - Jensen, Knud Jørgen

PY - 2013

Y1 - 2013

N2 - After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide Purities and for storage conditions are provided.

AB - After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide Purities and for storage conditions are provided.

KW - Peptide cleavage

KW - Side-chain deprotection

KW - Rink amide linker

KW - Wang linker

KW - Hydrazine benzoyl linker

KW - HMBA linker

U2 - 10.1007/978-1-62703-544-6_3

DO - 10.1007/978-1-62703-544-6_3

M3 - Book chapter

C2 - 23943477

SN - 978-1-62703-543-9

T3 - Methods in Molecular Biology

SP - 43

EP - 63

BT - Peptide synthesis and applications

A2 - Jensen, Knud J.

A2 - Shelton, Pernille T.

A2 - Pedersen, Søren L.

PB - Humana Press

ER -

ID: 118886988