Peptide release, side-chain deprotection, work-up, and isolation

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After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide Purities and for storage conditions are provided.
Original languageEnglish
Title of host publicationPeptide synthesis and applications
EditorsKnud J. Jensen, Pernille T. Shelton, Søren L. Pedersen
Number of pages21
PublisherHumana Press
Publication date2013
ISBN (Print)978-1-62703-543-9
ISBN (Electronic)978-1-62703-544-6
Publication statusPublished - 2013
SeriesMethods in Molecular Biology

ID: 118886988