Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease
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Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease. / Vainer, Ben; Nielsen, Ole H.; Hendel, Jakob; Horn, Thomas; Kirman, Irena.
In: Cytokine, Vol. 12, No. 10, 01.01.2000, p. 1531-1536.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease
AU - Vainer, Ben
AU - Nielsen, Ole H.
AU - Hendel, Jakob
AU - Horn, Thomas
AU - Kirman, Irena
PY - 2000/1/1
Y1 - 2000/1/1
N2 - A dysregulated local immune reaction with unbalanced cytokine expression seems essential in inflammatory bowel disease (IBD), i.e. ulcerative colitis (UC) and Crohn's disease (CD). Since the roles of interleukin (IL-)13 and IL-15 remain unclear, this study aimed at studying intestinal expression of IL-13 and IL-15 in IBD. Methods: In colonic biopsies from 24 UC, 18 CD, and 12 controls IL-13 and IL-15 were measured using ELISA, and their gene expressions were assessed by RT-PCR. Leukocytes were visualised histochemically. Results: Concentrations of IL-13 were decreased in UC (median 56 pg/mg tissue; interquartile range 30-99 pg/mg) compared to CD (82 pg/mg tissue; 41-122; P=0.004) and controls (83 pg/mg tissue; 18-134; P>0.05), and lower in active UC (53 pg/mg tissue; 33-96) than in inactive UC (80 pg/mg tissue; 65-99; P=0.02). IL-15 concentrations were higher in CD patients (34. pg/mg tissue; 24-53) as compared to controls (20 pg/mg tissue; 15-21; P=0.001) whilst being 22 pg/mg tissue (15-32) in UC. IL-13 mRNA and IL-15 mRNA were detected in 20% and 15%, respectively. Infiltration of leukocytes correlated inversely with IL-13 levels (P=0.02). Conclusion: Active UC is associated with decreased colonic IL-13 suggesting that IL-13 levels are diminished as a part of UC exacerbations, or that exacerbations follow active downregulation of IL-13. (C) 2000 Academic Press.
AB - A dysregulated local immune reaction with unbalanced cytokine expression seems essential in inflammatory bowel disease (IBD), i.e. ulcerative colitis (UC) and Crohn's disease (CD). Since the roles of interleukin (IL-)13 and IL-15 remain unclear, this study aimed at studying intestinal expression of IL-13 and IL-15 in IBD. Methods: In colonic biopsies from 24 UC, 18 CD, and 12 controls IL-13 and IL-15 were measured using ELISA, and their gene expressions were assessed by RT-PCR. Leukocytes were visualised histochemically. Results: Concentrations of IL-13 were decreased in UC (median 56 pg/mg tissue; interquartile range 30-99 pg/mg) compared to CD (82 pg/mg tissue; 41-122; P=0.004) and controls (83 pg/mg tissue; 18-134; P>0.05), and lower in active UC (53 pg/mg tissue; 33-96) than in inactive UC (80 pg/mg tissue; 65-99; P=0.02). IL-15 concentrations were higher in CD patients (34. pg/mg tissue; 24-53) as compared to controls (20 pg/mg tissue; 15-21; P=0.001) whilst being 22 pg/mg tissue (15-32) in UC. IL-13 mRNA and IL-15 mRNA were detected in 20% and 15%, respectively. Infiltration of leukocytes correlated inversely with IL-13 levels (P=0.02). Conclusion: Active UC is associated with decreased colonic IL-13 suggesting that IL-13 levels are diminished as a part of UC exacerbations, or that exacerbations follow active downregulation of IL-13. (C) 2000 Academic Press.
KW - Colon
KW - Cytokines
KW - Inflammatory bowel disease
KW - Interleukin-13
KW - Interleukin-15
UR - http://www.scopus.com/inward/record.url?scp=0033790206&partnerID=8YFLogxK
U2 - 10.1006/cyto.2000.0744
DO - 10.1006/cyto.2000.0744
M3 - Journal article
C2 - 11023669
AN - SCOPUS:0033790206
VL - 12
SP - 1531
EP - 1536
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 10
ER -
ID: 218717371