Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease

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Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease. / Vainer, Ben; Nielsen, Ole H.; Hendel, Jakob; Horn, Thomas; Kirman, Irena.

In: Cytokine, Vol. 12, No. 10, 01.01.2000, p. 1531-1536.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Vainer, B, Nielsen, OH, Hendel, J, Horn, T & Kirman, I 2000, 'Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease', Cytokine, vol. 12, no. 10, pp. 1531-1536. https://doi.org/10.1006/cyto.2000.0744

APA

Vainer, B., Nielsen, O. H., Hendel, J., Horn, T., & Kirman, I. (2000). Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease. Cytokine, 12(10), 1531-1536. https://doi.org/10.1006/cyto.2000.0744

Vancouver

Vainer B, Nielsen OH, Hendel J, Horn T, Kirman I. Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease. Cytokine. 2000 Jan 1;12(10):1531-1536. https://doi.org/10.1006/cyto.2000.0744

Author

Vainer, Ben ; Nielsen, Ole H. ; Hendel, Jakob ; Horn, Thomas ; Kirman, Irena. / Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease. In: Cytokine. 2000 ; Vol. 12, No. 10. pp. 1531-1536.

Bibtex

@article{e16b841233bc432d867bbf0404a68f6f,
title = "Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease",
abstract = "A dysregulated local immune reaction with unbalanced cytokine expression seems essential in inflammatory bowel disease (IBD), i.e. ulcerative colitis (UC) and Crohn's disease (CD). Since the roles of interleukin (IL-)13 and IL-15 remain unclear, this study aimed at studying intestinal expression of IL-13 and IL-15 in IBD. Methods: In colonic biopsies from 24 UC, 18 CD, and 12 controls IL-13 and IL-15 were measured using ELISA, and their gene expressions were assessed by RT-PCR. Leukocytes were visualised histochemically. Results: Concentrations of IL-13 were decreased in UC (median 56 pg/mg tissue; interquartile range 30-99 pg/mg) compared to CD (82 pg/mg tissue; 41-122; P=0.004) and controls (83 pg/mg tissue; 18-134; P>0.05), and lower in active UC (53 pg/mg tissue; 33-96) than in inactive UC (80 pg/mg tissue; 65-99; P=0.02). IL-15 concentrations were higher in CD patients (34. pg/mg tissue; 24-53) as compared to controls (20 pg/mg tissue; 15-21; P=0.001) whilst being 22 pg/mg tissue (15-32) in UC. IL-13 mRNA and IL-15 mRNA were detected in 20{\%} and 15{\%}, respectively. Infiltration of leukocytes correlated inversely with IL-13 levels (P=0.02). Conclusion: Active UC is associated with decreased colonic IL-13 suggesting that IL-13 levels are diminished as a part of UC exacerbations, or that exacerbations follow active downregulation of IL-13. (C) 2000 Academic Press.",
keywords = "Colon, Cytokines, Inflammatory bowel disease, Interleukin-13, Interleukin-15",
author = "Ben Vainer and Nielsen, {Ole H.} and Jakob Hendel and Thomas Horn and Irena Kirman",
year = "2000",
month = "1",
day = "1",
doi = "10.1006/cyto.2000.0744",
language = "English",
volume = "12",
pages = "1531--1536",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press",
number = "10",

}

RIS

TY - JOUR

T1 - Colonic expression and synthesis of interleukin 13 and interleukin 15 in inflammatory bowel disease

AU - Vainer, Ben

AU - Nielsen, Ole H.

AU - Hendel, Jakob

AU - Horn, Thomas

AU - Kirman, Irena

PY - 2000/1/1

Y1 - 2000/1/1

N2 - A dysregulated local immune reaction with unbalanced cytokine expression seems essential in inflammatory bowel disease (IBD), i.e. ulcerative colitis (UC) and Crohn's disease (CD). Since the roles of interleukin (IL-)13 and IL-15 remain unclear, this study aimed at studying intestinal expression of IL-13 and IL-15 in IBD. Methods: In colonic biopsies from 24 UC, 18 CD, and 12 controls IL-13 and IL-15 were measured using ELISA, and their gene expressions were assessed by RT-PCR. Leukocytes were visualised histochemically. Results: Concentrations of IL-13 were decreased in UC (median 56 pg/mg tissue; interquartile range 30-99 pg/mg) compared to CD (82 pg/mg tissue; 41-122; P=0.004) and controls (83 pg/mg tissue; 18-134; P>0.05), and lower in active UC (53 pg/mg tissue; 33-96) than in inactive UC (80 pg/mg tissue; 65-99; P=0.02). IL-15 concentrations were higher in CD patients (34. pg/mg tissue; 24-53) as compared to controls (20 pg/mg tissue; 15-21; P=0.001) whilst being 22 pg/mg tissue (15-32) in UC. IL-13 mRNA and IL-15 mRNA were detected in 20% and 15%, respectively. Infiltration of leukocytes correlated inversely with IL-13 levels (P=0.02). Conclusion: Active UC is associated with decreased colonic IL-13 suggesting that IL-13 levels are diminished as a part of UC exacerbations, or that exacerbations follow active downregulation of IL-13. (C) 2000 Academic Press.

AB - A dysregulated local immune reaction with unbalanced cytokine expression seems essential in inflammatory bowel disease (IBD), i.e. ulcerative colitis (UC) and Crohn's disease (CD). Since the roles of interleukin (IL-)13 and IL-15 remain unclear, this study aimed at studying intestinal expression of IL-13 and IL-15 in IBD. Methods: In colonic biopsies from 24 UC, 18 CD, and 12 controls IL-13 and IL-15 were measured using ELISA, and their gene expressions were assessed by RT-PCR. Leukocytes were visualised histochemically. Results: Concentrations of IL-13 were decreased in UC (median 56 pg/mg tissue; interquartile range 30-99 pg/mg) compared to CD (82 pg/mg tissue; 41-122; P=0.004) and controls (83 pg/mg tissue; 18-134; P>0.05), and lower in active UC (53 pg/mg tissue; 33-96) than in inactive UC (80 pg/mg tissue; 65-99; P=0.02). IL-15 concentrations were higher in CD patients (34. pg/mg tissue; 24-53) as compared to controls (20 pg/mg tissue; 15-21; P=0.001) whilst being 22 pg/mg tissue (15-32) in UC. IL-13 mRNA and IL-15 mRNA were detected in 20% and 15%, respectively. Infiltration of leukocytes correlated inversely with IL-13 levels (P=0.02). Conclusion: Active UC is associated with decreased colonic IL-13 suggesting that IL-13 levels are diminished as a part of UC exacerbations, or that exacerbations follow active downregulation of IL-13. (C) 2000 Academic Press.

KW - Colon

KW - Cytokines

KW - Inflammatory bowel disease

KW - Interleukin-13

KW - Interleukin-15

UR - http://www.scopus.com/inward/record.url?scp=0033790206&partnerID=8YFLogxK

U2 - 10.1006/cyto.2000.0744

DO - 10.1006/cyto.2000.0744

M3 - Journal article

C2 - 11023669

AN - SCOPUS:0033790206

VL - 12

SP - 1531

EP - 1536

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 10

ER -

ID: 218717371