Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

Research output: Contribution to journalJournal articleResearchpeer-review


Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.

Original languageEnglish
Article number2284
JournalNature Communications
Issue number1
Number of pages10
Publication statusPublished - 1 Dec 2020

Number of downloads are based on statistics from Google Scholar and

No data available

ID: 245323680