Weaning triggers a maturation step of pancreatic β cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Weaning triggers a maturation step of pancreatic β cells. / Stolovich-Rain, Miri; Enk, Jonatan; Vikesa, Jonas; Nielsen, Finn Cilius; Saada, Ann; Glaser, Benjamin; Dor, Yuval.

In: Developmental Cell, Vol. 32, No. 5, 09.03.2015, p. 535-45.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Stolovich-Rain, M, Enk, J, Vikesa, J, Nielsen, FC, Saada, A, Glaser, B & Dor, Y 2015, 'Weaning triggers a maturation step of pancreatic β cells', Developmental Cell, vol. 32, no. 5, pp. 535-45. https://doi.org/10.1016/j.devcel.2015.01.002

APA

Stolovich-Rain, M., Enk, J., Vikesa, J., Nielsen, F. C., Saada, A., Glaser, B., & Dor, Y. (2015). Weaning triggers a maturation step of pancreatic β cells. Developmental Cell, 32(5), 535-45. https://doi.org/10.1016/j.devcel.2015.01.002

Vancouver

Stolovich-Rain M, Enk J, Vikesa J, Nielsen FC, Saada A, Glaser B et al. Weaning triggers a maturation step of pancreatic β cells. Developmental Cell. 2015 Mar 9;32(5):535-45. https://doi.org/10.1016/j.devcel.2015.01.002

Author

Stolovich-Rain, Miri ; Enk, Jonatan ; Vikesa, Jonas ; Nielsen, Finn Cilius ; Saada, Ann ; Glaser, Benjamin ; Dor, Yuval. / Weaning triggers a maturation step of pancreatic β cells. In: Developmental Cell. 2015 ; Vol. 32, No. 5. pp. 535-45.

Bibtex

@article{23f86dcd6fd9416c812207c1bd3f8225,
title = "Weaning triggers a maturation step of pancreatic β cells",
abstract = "Because tissue regeneration deteriorates with age, it is generally assumed that the younger the animal, the better it compensates for tissue damage. We have examined the effect of young age on compensatory proliferation of pancreatic β cells in vivo. Surprisingly, β cells in suckling mice fail to enter the cell division cycle in response to a diabetogenic injury or increased glycolysis. The potential of β cells for compensatory proliferation is acquired following premature weaning to normal chow, but not to a diet mimicking maternal milk. In addition, weaning coincides with enhanced glucose-stimulated oxidative phosphorylation and insulin secretion from islets. Transcriptome analysis reveals that weaning increases the expression of genes involved in replication licensing, suggesting a mechanism for increased responsiveness to the mitogenic activity of high glucose. We propose that weaning triggers a discrete maturation step of β cells, elevating both the mitogenic and secretory response to glucose.",
keywords = "Animals, Apoptosis, Biomarkers, Blotting, Western, Cell Proliferation, Cells, Cultured, Female, Gene Expression Profiling, Glucose, Hypoglycemic Agents, Immunoenzyme Techniques, Insulin, Insulin-Secreting Cells, Islets of Langerhans, Male, Mice, Mice, Inbred ICR, Mice, Transgenic, Oligonucleotide Array Sequence Analysis, RNA, Messenger, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Weaning",
author = "Miri Stolovich-Rain and Jonatan Enk and Jonas Vikesa and Nielsen, {Finn Cilius} and Ann Saada and Benjamin Glaser and Yuval Dor",
note = "Copyright {\textcopyright} 2015 Elsevier Inc. All rights reserved.",
year = "2015",
month = mar,
day = "9",
doi = "10.1016/j.devcel.2015.01.002",
language = "English",
volume = "32",
pages = "535--45",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Weaning triggers a maturation step of pancreatic β cells

AU - Stolovich-Rain, Miri

AU - Enk, Jonatan

AU - Vikesa, Jonas

AU - Nielsen, Finn Cilius

AU - Saada, Ann

AU - Glaser, Benjamin

AU - Dor, Yuval

N1 - Copyright © 2015 Elsevier Inc. All rights reserved.

PY - 2015/3/9

Y1 - 2015/3/9

N2 - Because tissue regeneration deteriorates with age, it is generally assumed that the younger the animal, the better it compensates for tissue damage. We have examined the effect of young age on compensatory proliferation of pancreatic β cells in vivo. Surprisingly, β cells in suckling mice fail to enter the cell division cycle in response to a diabetogenic injury or increased glycolysis. The potential of β cells for compensatory proliferation is acquired following premature weaning to normal chow, but not to a diet mimicking maternal milk. In addition, weaning coincides with enhanced glucose-stimulated oxidative phosphorylation and insulin secretion from islets. Transcriptome analysis reveals that weaning increases the expression of genes involved in replication licensing, suggesting a mechanism for increased responsiveness to the mitogenic activity of high glucose. We propose that weaning triggers a discrete maturation step of β cells, elevating both the mitogenic and secretory response to glucose.

AB - Because tissue regeneration deteriorates with age, it is generally assumed that the younger the animal, the better it compensates for tissue damage. We have examined the effect of young age on compensatory proliferation of pancreatic β cells in vivo. Surprisingly, β cells in suckling mice fail to enter the cell division cycle in response to a diabetogenic injury or increased glycolysis. The potential of β cells for compensatory proliferation is acquired following premature weaning to normal chow, but not to a diet mimicking maternal milk. In addition, weaning coincides with enhanced glucose-stimulated oxidative phosphorylation and insulin secretion from islets. Transcriptome analysis reveals that weaning increases the expression of genes involved in replication licensing, suggesting a mechanism for increased responsiveness to the mitogenic activity of high glucose. We propose that weaning triggers a discrete maturation step of β cells, elevating both the mitogenic and secretory response to glucose.

KW - Animals

KW - Apoptosis

KW - Biomarkers

KW - Blotting, Western

KW - Cell Proliferation

KW - Cells, Cultured

KW - Female

KW - Gene Expression Profiling

KW - Glucose

KW - Hypoglycemic Agents

KW - Immunoenzyme Techniques

KW - Insulin

KW - Insulin-Secreting Cells

KW - Islets of Langerhans

KW - Male

KW - Mice

KW - Mice, Inbred ICR

KW - Mice, Transgenic

KW - Oligonucleotide Array Sequence Analysis

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Weaning

U2 - 10.1016/j.devcel.2015.01.002

DO - 10.1016/j.devcel.2015.01.002

M3 - Journal article

C2 - 25662175

VL - 32

SP - 535

EP - 545

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 5

ER -

ID: 162858727