Validation of the AmpFlSTR® Identifiler® Direct PCR Amplification kit in a laboratory accredited according to the ISO17025 standard
Research output: Contribution to journal › Journal article › Research › peer-review
A total of 249 FTA® card samples were investigated with the Identifiler® Direct kit. PCR amplification was performed according to the manufacturer's protocol with modifications in the reaction volume (10-25μL), the number of PCR cycles (25-27) and the number of FTA-card punches (1-2). Full concordance was observed between the results obtained with the Identifiler® Direct kit and the washed punches typed with the Identifiler® kit. The success rate with one 1.2mm punch in a 15μL PCR reaction volume and 25 PCR-cycles was 93.3%. After retyping of the failed samples, the combined success rate was 98.7%. Overall, the quality of the results was reduced when the number of punches was increased or the PCR reaction volume was decreased. The Identifiler® Direct master mix may be used in combination with other primer sets for direct amplification of other loci, e.g. the 49 SNPs using the SNPforID multiplex assay.
| Original language | English |
|---|---|
| Journal | Forensic Science International: Genetics Supplement Series |
| Volume | 3 |
| Issue number | 1 |
| Pages (from-to) | e165-e166 |
| ISSN | 1875-1768 |
| DOIs | |
| Publication status | Published - Dec 2011 |
- Crime DNA reference samples, FTA card, Identifiler Direct Amplification kit, SNPforID multiplex assay, STR typing
Research areas
ID: 307818588