Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain. / Dreisig, Karin; Degn, Matilda; Sund, Louise; Hadaczek, Piotr; Samaranch, Lluis; San Sebastian, Waldy; Bankiewicz, Krystof; Rahbek Kornum, Birgitte.

In: Journal of Chemical Neuroanatomy, Vol. 78, 12.2016, p. 25-33.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dreisig, K, Degn, M, Sund, L, Hadaczek, P, Samaranch, L, San Sebastian, W, Bankiewicz, K & Rahbek Kornum, B 2016, 'Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain', Journal of Chemical Neuroanatomy, vol. 78, pp. 25-33. https://doi.org/10.1016/j.jchemneu.2016.08.002

APA

Dreisig, K., Degn, M., Sund, L., Hadaczek, P., Samaranch, L., San Sebastian, W., Bankiewicz, K., & Rahbek Kornum, B. (2016). Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain. Journal of Chemical Neuroanatomy, 78, 25-33. https://doi.org/10.1016/j.jchemneu.2016.08.002

Vancouver

Dreisig K, Degn M, Sund L, Hadaczek P, Samaranch L, San Sebastian W et al. Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain. Journal of Chemical Neuroanatomy. 2016 Dec;78:25-33. https://doi.org/10.1016/j.jchemneu.2016.08.002

Author

Dreisig, Karin ; Degn, Matilda ; Sund, Louise ; Hadaczek, Piotr ; Samaranch, Lluis ; San Sebastian, Waldy ; Bankiewicz, Krystof ; Rahbek Kornum, Birgitte. / Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain. In: Journal of Chemical Neuroanatomy. 2016 ; Vol. 78. pp. 25-33.

Bibtex

@article{39c6c2b926414d1b804899bebe800a8c,

title = "Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain",

abstract = "Focus on the purinergic receptor P2Y11 has increased following the finding of an association between the sleep disorder narcolepsy and a genetic variant in P2RY11 causing decreased gene expression. Narcolepsy is believed to arise from an autoimmune destruction of the hypothalamic neurons that produce the neuropeptide hypocretin/orexin. It is unknown how a decrease in expression of P2Y11 might contribute to an autoimmune reaction towards the hypocretin neurons and the development of narcolepsy. To advance narcolepsy research it is therefore extremely important to determine the neuroanatomical localization of P2Y11 in the brain with particular emphasis on the hypocretin neurons. In this article we used western blot, staining of blood smears, and flow cytometry to select two antibodies for immunohistochemical staining of macaque monkey brain. Staining was seen in neuron-like structures in cortical and hypothalamic regions. Rats do not have a gene orthologue to the P2Y11 receptor and therefore rat brain was used as negative control tissue. The chromogenic signal observed in macaque monkey brain in neurons was not considered reliable, because the antibodies stained rat brain in a similar distribution pattern. Hence, the neuroanatomical localization of the P2Y11 receptor remains undetermined due to the lack of specific P2Y11 antibodies for brain immunohistochemistry.",

keywords = "Animals, Cerebellum/metabolism, Hypothalamus/metabolism, Immunohistochemistry/methods, Macaca, Neurons/metabolism, Prefrontal Cortex/metabolism, Rats, Receptors, Purinergic P2/metabolism",

author = "Karin Dreisig and Matilda Degn and Louise Sund and Piotr Hadaczek and Lluis Samaranch and {San Sebastian}, Waldy and Krystof Bankiewicz and {Rahbek Kornum}, Birgitte",

year = "2016",

month = dec,

doi = "10.1016/j.jchemneu.2016.08.002",

language = "English",

volume = "78",

pages = "25--33",

journal = "Journal of Chemical Neuroanatomy",

issn = "0891-0618",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Validation of antibodies for neuroanatomical localization of the P2Y receptor in macaque brain

AU - Dreisig, Karin

AU - Degn, Matilda

AU - Sund, Louise

AU - Hadaczek, Piotr

AU - Samaranch, Lluis

AU - San Sebastian, Waldy

AU - Bankiewicz, Krystof

AU - Rahbek Kornum, Birgitte

PY - 2016/12

Y1 - 2016/12

N2 - Focus on the purinergic receptor P2Y11 has increased following the finding of an association between the sleep disorder narcolepsy and a genetic variant in P2RY11 causing decreased gene expression. Narcolepsy is believed to arise from an autoimmune destruction of the hypothalamic neurons that produce the neuropeptide hypocretin/orexin. It is unknown how a decrease in expression of P2Y11 might contribute to an autoimmune reaction towards the hypocretin neurons and the development of narcolepsy. To advance narcolepsy research it is therefore extremely important to determine the neuroanatomical localization of P2Y11 in the brain with particular emphasis on the hypocretin neurons. In this article we used western blot, staining of blood smears, and flow cytometry to select two antibodies for immunohistochemical staining of macaque monkey brain. Staining was seen in neuron-like structures in cortical and hypothalamic regions. Rats do not have a gene orthologue to the P2Y11 receptor and therefore rat brain was used as negative control tissue. The chromogenic signal observed in macaque monkey brain in neurons was not considered reliable, because the antibodies stained rat brain in a similar distribution pattern. Hence, the neuroanatomical localization of the P2Y11 receptor remains undetermined due to the lack of specific P2Y11 antibodies for brain immunohistochemistry.

AB - Focus on the purinergic receptor P2Y11 has increased following the finding of an association between the sleep disorder narcolepsy and a genetic variant in P2RY11 causing decreased gene expression. Narcolepsy is believed to arise from an autoimmune destruction of the hypothalamic neurons that produce the neuropeptide hypocretin/orexin. It is unknown how a decrease in expression of P2Y11 might contribute to an autoimmune reaction towards the hypocretin neurons and the development of narcolepsy. To advance narcolepsy research it is therefore extremely important to determine the neuroanatomical localization of P2Y11 in the brain with particular emphasis on the hypocretin neurons. In this article we used western blot, staining of blood smears, and flow cytometry to select two antibodies for immunohistochemical staining of macaque monkey brain. Staining was seen in neuron-like structures in cortical and hypothalamic regions. Rats do not have a gene orthologue to the P2Y11 receptor and therefore rat brain was used as negative control tissue. The chromogenic signal observed in macaque monkey brain in neurons was not considered reliable, because the antibodies stained rat brain in a similar distribution pattern. Hence, the neuroanatomical localization of the P2Y11 receptor remains undetermined due to the lack of specific P2Y11 antibodies for brain immunohistochemistry.

KW - Animals

KW - Cerebellum/metabolism

KW - Hypothalamus/metabolism

KW - Immunohistochemistry/methods

KW - Macaca

KW - Neurons/metabolism

KW - Prefrontal Cortex/metabolism

KW - Rats

KW - Receptors, Purinergic P2/metabolism

U2 - 10.1016/j.jchemneu.2016.08.002

DO - 10.1016/j.jchemneu.2016.08.002

M3 - Journal article

C2 - 27515691

VL - 78

SP - 25

EP - 33

JO - Journal of Chemical Neuroanatomy

JF - Journal of Chemical Neuroanatomy

SN - 0891-0618

ER -

ID: 196169169