Utility of X-chromosome SNPs in relationship testing

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Standard

Utility of X-chromosome SNPs in relationship testing. / Tomas, Carmen; Sanchez, Juan Jose; Castro, J.A.; Børsting, C.; Morling, Niels.

Progress in Forensic Genetics 12: Proceedings of the 22nd International ISFG Congress. ed. / Niels Morling. Vol. 1 Elsevier, 2008. p. 528-530 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Harvard

Tomas, C, Sanchez, JJ, Castro, JA, Børsting, C & Morling, N 2008, Utility of X-chromosome SNPs in relationship testing. in N Morling (ed.), Progress in Forensic Genetics 12: Proceedings of the 22nd International ISFG Congress. vol. 1, Elsevier, Forensic Science International: Genetics Supplement Series, no. 1, vol. 1, pp. 528-530, 22nd International ISFG Congress, Copenhagen, Denmark, 21/08/2007.

APA

Tomas, C., Sanchez, J. J., Castro, J. A., Børsting, C., & Morling, N. (2008). Utility of X-chromosome SNPs in relationship testing. In N. Morling (Ed.), Progress in Forensic Genetics 12: Proceedings of the 22nd International ISFG Congress (Vol. 1, pp. 528-530). Elsevier. Forensic Science International: Genetics Supplement Series Vol. 1 No. 1

Vancouver

Tomas C, Sanchez JJ, Castro JA, Børsting C, Morling N. Utility of X-chromosome SNPs in relationship testing. In Morling N, editor, Progress in Forensic Genetics 12: Proceedings of the 22nd International ISFG Congress. Vol. 1. Elsevier. 2008. p. 528-530. (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 1).

Author

Tomas, Carmen ; Sanchez, Juan Jose ; Castro, J.A. ; Børsting, C. ; Morling, Niels. / Utility of X-chromosome SNPs in relationship testing. Progress in Forensic Genetics 12: Proceedings of the 22nd International ISFG Congress. editor / Niels Morling. Vol. 1 Elsevier, 2008. pp. 528-530 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 1).

Bibtex

@inproceedings{67c6dcc0a2c011debc73000ea68e967b,
title = "Utility of X-chromosome SNPs in relationship testing",
abstract = "X-chromosome markers may complement the results obtained from other genetic markers in complex relationship cases. Until now, reports on relationship testing using X-chromosome markers have mainly included data of short tandem repeats (STRs) while little data on single nucleotide polymorphisms (SNPs) in relationship testing have been published. We selected 25 highly polymorphic biallelic SNPs distributed through the human X-chromosome. One 25-plex PCR reaction and one 25-plex single base extension (SNaPshot) reaction were developed. The maximum size of the PCR products was 120{\'a}bp and the size of the SBE primers varied between 18 and 85 nucleotides. We analyzed the allele and haplotype frequencies in 1078 unrelated males. All the SNPs were polymorphic and the lowest minor allele frequency was 0.103. All the haplotypes were unique. The forensic parameters were calculated in the Danish and Somali populations. In the Danish population (N{\'a}={\'a}93), the power of discrimination (PD) in females was one in 4.4{\'a}{\^I}{\'a}109 individuals and the PD in males was one in 2.6{\'a}{\^I}{\'a}106. The PD in Somalis (N{\'a}={\'a}91) was one in 2.7{\'a}{\^I}{\'a}109 in females and one in 1.7{\'a}{\^I}{\'a}106 in males. Finally, we present an example of how the 25 X-chromosome SNP-multiplex may help to solve a complex immigration case",
author = "Carmen Tomas and Sanchez, {Juan Jose} and J.A. Castro and C. B{\o}rsting and Niels Morling",
year = "2008",
language = "English",
volume = "1",
series = "Forensic Science International: Genetics Supplement Series",
publisher = "Elsevier",
number = "1",
pages = "528--530",
editor = "Niels Morling",
booktitle = "Progress in Forensic Genetics 12",
address = "Netherlands",
note = "null ; Conference date: 21-08-2007 Through 25-08-2007",

}

RIS

TY - GEN

T1 - Utility of X-chromosome SNPs in relationship testing

AU - Tomas, Carmen

AU - Sanchez, Juan Jose

AU - Castro, J.A.

AU - Børsting, C.

AU - Morling, Niels

PY - 2008

Y1 - 2008

N2 - X-chromosome markers may complement the results obtained from other genetic markers in complex relationship cases. Until now, reports on relationship testing using X-chromosome markers have mainly included data of short tandem repeats (STRs) while little data on single nucleotide polymorphisms (SNPs) in relationship testing have been published. We selected 25 highly polymorphic biallelic SNPs distributed through the human X-chromosome. One 25-plex PCR reaction and one 25-plex single base extension (SNaPshot) reaction were developed. The maximum size of the PCR products was 120ábp and the size of the SBE primers varied between 18 and 85 nucleotides. We analyzed the allele and haplotype frequencies in 1078 unrelated males. All the SNPs were polymorphic and the lowest minor allele frequency was 0.103. All the haplotypes were unique. The forensic parameters were calculated in the Danish and Somali populations. In the Danish population (Ná=á93), the power of discrimination (PD) in females was one in 4.4áÎá109 individuals and the PD in males was one in 2.6áÎá106. The PD in Somalis (Ná=á91) was one in 2.7áÎá109 in females and one in 1.7áÎá106 in males. Finally, we present an example of how the 25 X-chromosome SNP-multiplex may help to solve a complex immigration case

AB - X-chromosome markers may complement the results obtained from other genetic markers in complex relationship cases. Until now, reports on relationship testing using X-chromosome markers have mainly included data of short tandem repeats (STRs) while little data on single nucleotide polymorphisms (SNPs) in relationship testing have been published. We selected 25 highly polymorphic biallelic SNPs distributed through the human X-chromosome. One 25-plex PCR reaction and one 25-plex single base extension (SNaPshot) reaction were developed. The maximum size of the PCR products was 120ábp and the size of the SBE primers varied between 18 and 85 nucleotides. We analyzed the allele and haplotype frequencies in 1078 unrelated males. All the SNPs were polymorphic and the lowest minor allele frequency was 0.103. All the haplotypes were unique. The forensic parameters were calculated in the Danish and Somali populations. In the Danish population (Ná=á93), the power of discrimination (PD) in females was one in 4.4áÎá109 individuals and the PD in males was one in 2.6áÎá106. The PD in Somalis (Ná=á91) was one in 2.7áÎá109 in females and one in 1.7áÎá106 in males. Finally, we present an example of how the 25 X-chromosome SNP-multiplex may help to solve a complex immigration case

M3 - Article in proceedings

VL - 1

T3 - Forensic Science International: Genetics Supplement Series

SP - 528

EP - 530

BT - Progress in Forensic Genetics 12

A2 - Morling, Niels

PB - Elsevier

Y2 - 21 August 2007 through 25 August 2007

ER -

ID: 14465725