Used Nasogastric Feeding Tubes from Neonates Contain Infant-Specific Bacterial Profiles
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Used Nasogastric Feeding Tubes from Neonates Contain Infant-Specific Bacterial Profiles. / Meinich Juhl, Sandra; Angeliki Krogfelt, Karen; Kot, Witold; Sandris Nielsen, Dennis; Krych, Lukasz.
In: Microorganisms, Vol. 11, No. 6, 1365, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Used Nasogastric Feeding Tubes from Neonates Contain Infant-Specific Bacterial Profiles
AU - Meinich Juhl, Sandra
AU - Angeliki Krogfelt, Karen
AU - Kot, Witold
AU - Sandris Nielsen, Dennis
AU - Krych, Lukasz
N1 - Publisher Copyright: © 2023 by the authors.
PY - 2023
Y1 - 2023
N2 - Nasogastric feeding tubes (NG-tubes) from neonates contain potentially pathogenic bacteria. Using culture-based techniques, we have previously determined that the usage duration of NG-tubes did not impact the colonization of the nasogastric tubes. In the present study, we performed 16S rRNA gene amplicon sequencing to evaluate the microbial profile of 94 used nasogastric tubes collected from a single neonatal intensive care unit. Using culture-based whole genome sequencing, we as-sessed whether the same strain persisted in NG-tubes collected from the same neonate across different time-points. We found that the most commonly occurring Gram-negative bacteria were Enterobacteriaceae, Klebsiella and Serratia, while the most common Gram-positive bacteria were staphylococci and streptococci. The microbiota of the NG-feeding tube was overall infant-specific, rather than dependent on the duration of use. Furthermore, we determined that reoccurring species from the individual infant represented the same strain and that several strains were common for more than one infant. Our findings indicate that bacterial profiles found in NG-tubes of neonates are host-specific, not dependent on the duration of use and strongly influenced by the environment.
AB - Nasogastric feeding tubes (NG-tubes) from neonates contain potentially pathogenic bacteria. Using culture-based techniques, we have previously determined that the usage duration of NG-tubes did not impact the colonization of the nasogastric tubes. In the present study, we performed 16S rRNA gene amplicon sequencing to evaluate the microbial profile of 94 used nasogastric tubes collected from a single neonatal intensive care unit. Using culture-based whole genome sequencing, we as-sessed whether the same strain persisted in NG-tubes collected from the same neonate across different time-points. We found that the most commonly occurring Gram-negative bacteria were Enterobacteriaceae, Klebsiella and Serratia, while the most common Gram-positive bacteria were staphylococci and streptococci. The microbiota of the NG-feeding tube was overall infant-specific, rather than dependent on the duration of use. Furthermore, we determined that reoccurring species from the individual infant represented the same strain and that several strains were common for more than one infant. Our findings indicate that bacterial profiles found in NG-tubes of neonates are host-specific, not dependent on the duration of use and strongly influenced by the environment.
KW - colonization
KW - contamination
KW - feeding tube
KW - infant
KW - infection risk
KW - neonatal intensive care unit
U2 - 10.3390/microorganisms11061365
DO - 10.3390/microorganisms11061365
M3 - Journal article
C2 - 37374868
AN - SCOPUS:85163831614
VL - 11
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 6
M1 - 1365
ER -
ID: 360686047