Uncovering new substrates for Aurora A kinase
Research output: Contribution to journal › Journal article › Research › peer-review
Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.
Original language | English |
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Journal | EMBO Reports |
Volume | 11 |
Issue number | 12 |
Pages (from-to) | 977-984 |
Number of pages | 8 |
ISSN | 1469-221X |
DOIs | |
Publication status | Published - 2010 |
Externally published | Yes |
- Amino Acid Sequence, Aurora Kinases, Computational Biology, Humans, Microtubule-Associated Proteins/chemistry, Mitosis, Models, Biological, Molecular Sequence Data, Phosphorylation, Phosphoserine/metabolism, Protein-Serine-Threonine Kinases/metabolism, Reproducibility of Results, Substrate Specificity
Research areas
ID: 203256455