Typing of Y chromosome SNPs with multiplex PCR methods

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Standard

Typing of Y chromosome SNPs with multiplex PCR methods. / Sanchez Sanchez, Juan Jose; Børsting, Claus; Morling, Niels.

In: Methods in Molecular Biology, Vol. 297, 2005, p. 209-28.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sanchez Sanchez, JJ, Børsting, C & Morling, N 2005, 'Typing of Y chromosome SNPs with multiplex PCR methods', Methods in Molecular Biology, vol. 297, pp. 209-28.

APA

Sanchez Sanchez, J. J., Børsting, C., & Morling, N. (2005). Typing of Y chromosome SNPs with multiplex PCR methods. Methods in Molecular Biology, 297, 209-28.

Vancouver

Sanchez Sanchez JJ, Børsting C, Morling N. Typing of Y chromosome SNPs with multiplex PCR methods. Methods in Molecular Biology. 2005;297:209-28.

Author

Sanchez Sanchez, Juan Jose ; Børsting, Claus ; Morling, Niels. / Typing of Y chromosome SNPs with multiplex PCR methods. In: Methods in Molecular Biology. 2005 ; Vol. 297. pp. 209-28.

Bibtex

@article{27ef202097ae11de8bc9000ea68e967b,
title = "Typing of Y chromosome SNPs with multiplex PCR methods",
abstract = "We describe a method for the simultaneous typing of Y-chromosome single nucleotide polymorphism (SNP) markers by means of multiplex polymerase chain reaction (PCR) strategies that allow the detection of 35 Y chromosome SNPs on 25 amplicons from 100 to 200 pg of chromosomal deoxyribonucleic acid (DNA). Multiplex PCR amplification of the DNA was performed with slight modifications of standard PCR conditions. Single-base extension (SBE) was performed using the SNaPshot kit containing fluorescently labeled ddNTPs. The extended primers were detected on an ABI 3100 sequencer. The most important factors for the creation of larger SNP typing PCR multiplexes include careful selection of primers for the primary amplification and the SBE reaction, use of DNA primers with homogenous composition, and balancing the primer concentrations for both the amplification and the SBE reactions.",
author = "{Sanchez Sanchez}, {Juan Jose} and Claus B{\o}rsting and Niels Morling",
note = "Keywords: Base Sequence; Chromosomes, Human, Y; DNA Primers; Electrophoresis, Polyacrylamide Gel; Humans; Polymerase Chain Reaction; Polymorphism, Single Nucleotide",
year = "2005",
language = "English",
volume = "297",
pages = "209--28",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Typing of Y chromosome SNPs with multiplex PCR methods

AU - Sanchez Sanchez, Juan Jose

AU - Børsting, Claus

AU - Morling, Niels

N1 - Keywords: Base Sequence; Chromosomes, Human, Y; DNA Primers; Electrophoresis, Polyacrylamide Gel; Humans; Polymerase Chain Reaction; Polymorphism, Single Nucleotide

PY - 2005

Y1 - 2005

N2 - We describe a method for the simultaneous typing of Y-chromosome single nucleotide polymorphism (SNP) markers by means of multiplex polymerase chain reaction (PCR) strategies that allow the detection of 35 Y chromosome SNPs on 25 amplicons from 100 to 200 pg of chromosomal deoxyribonucleic acid (DNA). Multiplex PCR amplification of the DNA was performed with slight modifications of standard PCR conditions. Single-base extension (SBE) was performed using the SNaPshot kit containing fluorescently labeled ddNTPs. The extended primers were detected on an ABI 3100 sequencer. The most important factors for the creation of larger SNP typing PCR multiplexes include careful selection of primers for the primary amplification and the SBE reaction, use of DNA primers with homogenous composition, and balancing the primer concentrations for both the amplification and the SBE reactions.

AB - We describe a method for the simultaneous typing of Y-chromosome single nucleotide polymorphism (SNP) markers by means of multiplex polymerase chain reaction (PCR) strategies that allow the detection of 35 Y chromosome SNPs on 25 amplicons from 100 to 200 pg of chromosomal deoxyribonucleic acid (DNA). Multiplex PCR amplification of the DNA was performed with slight modifications of standard PCR conditions. Single-base extension (SBE) was performed using the SNaPshot kit containing fluorescently labeled ddNTPs. The extended primers were detected on an ABI 3100 sequencer. The most important factors for the creation of larger SNP typing PCR multiplexes include careful selection of primers for the primary amplification and the SBE reaction, use of DNA primers with homogenous composition, and balancing the primer concentrations for both the amplification and the SBE reactions.

M3 - Journal article

C2 - 15570110

VL - 297

SP - 209

EP - 228

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 14145223