trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements

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trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements. / Drew, Jeff; Belsham, Graham J.

In: Journal of Virology, Vol. 68, No. 2, 02.1994, p. 697-703.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Drew, J & Belsham, GJ 1994, 'trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements', Journal of Virology, vol. 68, no. 2, pp. 697-703. https://doi.org/10.1128/jvi.68.2.697-703.1994

APA

Drew, J., & Belsham, G. J. (1994). trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements. Journal of Virology, 68(2), 697-703. https://doi.org/10.1128/jvi.68.2.697-703.1994

Vancouver

Drew J, Belsham GJ. trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements. Journal of Virology. 1994 Feb;68(2):697-703. https://doi.org/10.1128/jvi.68.2.697-703.1994

Author

Drew, Jeff ; Belsham, Graham J. / trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements. In: Journal of Virology. 1994 ; Vol. 68, No. 2. pp. 697-703.

Bibtex

@article{549d340fda63489799799cdca2ac5f72,
title = "trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements",
abstract = "A region of about 435 bases from the 5' noncoding region of foot-and- mouth disease virus RNA directs internal initiation of protein synthesis. This region, termed the internal ribosome entry site (IRES), is predicted to contain extensive secondary structure. Precise deletion of five predicted secondary structure features has been performed. The mutant IRES elements have been constructed into vectors which express bicistronic mRNAs and assayed within cells. Each of the modified IRES elements was defective in directing internal initiation when assayed alone. However, coexpression of an intact foot-and-mouth disease virus IRES complemented four of these defective elements to an efficiency of up to 80% of wild-type activity. No complementation was observed with the structurally analogous element from encephalomyocarditis virus. The role of RNA-RNA interactions in the function of the picornavirus IRES is discussed.",
author = "Jeff Drew and Belsham, {Graham J.}",
year = "1994",
month = feb,
doi = "10.1128/jvi.68.2.697-703.1994",
language = "English",
volume = "68",
pages = "697--703",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - trans Complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements

AU - Drew, Jeff

AU - Belsham, Graham J.

PY - 1994/2

Y1 - 1994/2

N2 - A region of about 435 bases from the 5' noncoding region of foot-and- mouth disease virus RNA directs internal initiation of protein synthesis. This region, termed the internal ribosome entry site (IRES), is predicted to contain extensive secondary structure. Precise deletion of five predicted secondary structure features has been performed. The mutant IRES elements have been constructed into vectors which express bicistronic mRNAs and assayed within cells. Each of the modified IRES elements was defective in directing internal initiation when assayed alone. However, coexpression of an intact foot-and-mouth disease virus IRES complemented four of these defective elements to an efficiency of up to 80% of wild-type activity. No complementation was observed with the structurally analogous element from encephalomyocarditis virus. The role of RNA-RNA interactions in the function of the picornavirus IRES is discussed.

AB - A region of about 435 bases from the 5' noncoding region of foot-and- mouth disease virus RNA directs internal initiation of protein synthesis. This region, termed the internal ribosome entry site (IRES), is predicted to contain extensive secondary structure. Precise deletion of five predicted secondary structure features has been performed. The mutant IRES elements have been constructed into vectors which express bicistronic mRNAs and assayed within cells. Each of the modified IRES elements was defective in directing internal initiation when assayed alone. However, coexpression of an intact foot-and-mouth disease virus IRES complemented four of these defective elements to an efficiency of up to 80% of wild-type activity. No complementation was observed with the structurally analogous element from encephalomyocarditis virus. The role of RNA-RNA interactions in the function of the picornavirus IRES is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0028177935&partnerID=8YFLogxK

U2 - 10.1128/jvi.68.2.697-703.1994

DO - 10.1128/jvi.68.2.697-703.1994

M3 - Journal article

C2 - 8289373

AN - SCOPUS:0028177935

VL - 68

SP - 697

EP - 703

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

ER -

ID: 381222004